Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides

Biodegradation in water: screening tests

Description of key information

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides(CAS No. 83968-28-9). The screening test inherent to the biodegradability of the substance was calculated using the software BIOWIN v4.10. The results indicate that Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochloride is not expected to be readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The predicted study for the test compound Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides(CAS No. 83968-28-9) and its read across substance were reviewed for the biodegradation end point which is summarized as below:

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides(CAS No. 83968-28-9). The screening test inherent to the biodegradability of the substance was calculated using the software BIOWIN v4.10. The results indicate that Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochloride is not expected to be readily biodegradable.

5-days Closed bottle test to determine biological oxygen demand and thereby % degradation during the test period was conducted for the read across substanceN, N- diethylaniline(CAS No.91-66-7)[UERLStudy Report, Sustainability Support Services (Europe) AB (Report no. UERL/BOD-005/91 -66 -7/2014), 2014]. The test system included control, test substance and reference substance. The concentration of test and reference substance (sodium acetate) chosen for the study were 2 mg/l & 5 mg/l respectively while that of inoculum (diluted soil suspension) was 0.5 ml/l. Further, ThOD (Theoretical oxygen demand) of test substance was determined by calculation/experimentally. % Degradation was calculated using the values of BOD and ThOD for test substance and reference substance. The BOD₅value of N,N- diethylaniline(CAS No.91-66-7) was observed to be 0.375 mgO₂/mg. ThOD was determined by calculation as 2.78 mgO₂/mg. Accordingly, the % degradation of the test substance after 5 days of incubation at 20°C according to closed bottle test was found to be 13.48%. Based on the results, the test item, under the test conditions, was found to be inherently biodegradable at 20°C over a period of 5 days.

 

 

In 2 weeks study (J-CHECK database, 1977) of biodegradation of N,N-diethylaniline, Concentration of inoculum i.e, sludge is 30 mg/l and initial test substance conc. used in the study is 100 mg/l. The percentage degradation of test substance was found to be 0% by BOD, and 1% by HPLC. Thus it is concluded that substance N,N-diethylaniline is found to be not readily biodegradable under test conditions.

Biodegradation screening test (J-CHECK, 2016) was conducted for 14 days (2 weeks) for evaluating the percentage biodegradability of the test substance. Concentration of inoculum i.e, sludge is 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 1.9% by BOD, 12.3% by TOC and 0% by GC. Thus, the substance N,N-Dimethylaniline was considered to be not readily biodegradable in water.

On the basis of the overall reported results of target and its read across substance, it can be concluded that the substance was found to be not-readily biodegradable in water.