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EC number: 946-245-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 438 (Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of (E)-3,4,5,6,6-pentamethylhept-3-en-2-one and 3,5,6,6-tetramethyl-4-methyleneheptan-2-one
- EC Number:
- 946-245-5
- Molecular formula:
- C12H22O
- IUPAC Name:
- Reaction mass of (E)-3,4,5,6,6-pentamethylhept-3-en-2-one and 3,5,6,6-tetramethyl-4-methyleneheptan-2-one
- Test material form:
- liquid
- Details on test material:
- According to ECHA communication with reference to Annotation number: SUB-C-2114629169-42-01/F, the substance ID for Koavone has been changed with EC# from 939-627-8 to 946-245-5, and the IUPAC name from Reaction mass of (3R,5R)-3,5,6,6-tetramethyl-4-methylideneheptan-2-one and (3R,5S)-3,5,6,6-tetramethyl-4-methylideneheptan-2-one and (E)-3,4,5,6,6-pentamethylhept-3-en-2-one to Reaction mass of (E)-3,4,5,6,6-pentamethylhept-3-en-2-one and 3,5,6,6-tetramethyl-4-methyleneheptan-2-one.
Constituent 1
Test animals / tissue source
- Species:
- other: chicken eyes (in vitro)
- Strain:
- other: ROSS (spring chickens)
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- 30 μL
- Duration of treatment / exposure:
- 10 seconds
- Duration of post- treatment incubation (in vitro):
- Ca. 0, 30, 75, 120, 180 and 240 minutes after treatment
- Number of animals or in vitro replicates:
- 3 eyes for the substance, 3 eyes for positive control and 1 eye for negative control
- Details on study design:
- Approximately 7 weeks old, male or female chickens were used as eye donors. Within 2 hours after kill, eyes were carefully dissected and placed in a superfusion apparatus. The chambers of the superfusion apparatus were temperature controlled at approximately 32 ºC. The eyes were examined with the slit-lamp microscope to ensure that they were not damaged. Corneal thickness was measured using the Depth Measuring Attachment No. 1. Eyes with a corneal thickness deviating more than 10% of the average corneal thickness of the eyes, eyes that showed opacity (score > 0.5), or were unacceptably scored with fluorescein (score higher than 0.5), or eyes showing any other signs of damage were rejected. The eyes were treated with 30 μLof the test substance for 10 seconds and rinsed with 20 mL saline. The eyes were examined at approximately 0, 30, 75, 120, 180 and 240 minutes after treatment using the following criteria:
Corneal swelling: expressed as a percentage (max. 100%): calculated as corneal thickness at time t minus corneal thickness at time 0, divided by corneal thickness at time 0 and multiplied by 100.
Corneal opacity:
0 = no opacity
0.5 = very faint opacity (very slight)
1 = scattered or diffuse areas, details of iris clearly visible (= slight)
2 = easily discernible translucent area, details of iris slightly obscured ( = moderate)
3 = severe corneal opacity, no specific details of iris visible, size of pupil barely discernible (= severe)
4 = complete corneal opacity, iris invisible (= very severe)
Fluorescein retention:
0 = no fluorescein retention
0.5 = very minor single cell staining ( = very slight)
1 = single cell staining cattered throughout the treated area of the cornea ( = slight)
2 = focal or confluent dense single cell staining (moderate)
3 = confluent large area of the cornea retaining fluorescein (= severe)
Fluorescein retention was only scored at approximately 30 minutes after treatment. All examinations were carried out with the slit-lamp microscope.
The scores were converted into the irritation categories according to the following scheme:
I = no effect; II = slight effect; III = moderate effect; IV = severe effect
Regulatory GHS Classification:
A: not irritating: 3 x Category I; 2 x Category I, 1 x Category II
B: slightly irritating: 3 x Category II; 2 x Category II, 1 x Category III; 1 x Category 1, 1 x Category II, 1 x Category III
C: moderately irritating (GHS Category 2A): 3 x Category III; 2 x Category III, 1 x Category II; 2 x Category III, 1 x Category I; 2 x Category I, 1 x Category IV; 2 x Category II, 1 x Category IV; 2 x Category III, 1 x Category IV; 1 x Category II, 1 x Category III, 1 x Category IV
D: severely irritating (GHS Category 1): 3 x Category IV; 2 x Category IV, 1 x Category III; 2 x Category IV, 1 x Category II; 2 x Category IV, 1 x Category I; immediate corneal opacity score 3 in at least 2 corneas; corneal opacity score 4 in at least 2 corneas; severe loosening of epithelium in at least one cornea
Irritation index was calculated as follows: maximum corneal swelling + maximum mean opacity (x 20) + mean fluorescein score (x 20)
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Remarks:
- mean
- Run / experiment:
- 240 minutes after treatment
- Value:
- 1
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Irritancy category II
- Irritation parameter:
- percent corneal swelling
- Remarks:
- mean
- Run / experiment:
- 240 minutes after treatment
- Value:
- 0
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Irritancy category I
- Irritation parameter:
- fluorescein retention score
- Remarks:
- mean
- Run / experiment:
- 240 minutes after treatment
- Value:
- 1
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Irritancy category II
- Other effects / acceptance of results:
- The following irritation categories were reported for the substance: I (swelling), II (opacity), II (fluorescin retention); irritation index 40
For saline (negative control): corneal swelling 0, corneal opacity 0, fluorescein retention 0
For 5% BAC (positive control): 29% corneal swelling, corneal opacity 3.0, fluorescein retention 3.0; irritation categories III, IV and IV, respectively; irritation index 149.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not irritating
- Remarks:
- in accordance with EU CLP (EC no 1272/2008 and its amendments)
- Conclusions:
- The substance caused no swelling of the cornea, slight corneal opacity and slight fluorescein retention in the in vitro Isolated Chicken Eye test (OECD TG 438). The substance is considered to be not irritating to the eyes.
- Executive summary:
The eye irritating properties of the substance were tested in vitro, in an Isolated Chicken Eye (ICE) test according to OECD TG 438 and in compliance with GLP. The test included a negative control (saline) and a positive control (5% benzalkonium chloride). The isolated chicken eyes were treated with of 30 μL of the substance for 10 seconds and then rinsed with 20 mL saline. Corneal swelling, corneal opacity and fluorescein retention were measured to disclose possible adverse effects. The substance caused no swelling of the cornea (0%), slight corneal opacity (score 1 out of max. 4) and slight fluorescein retention (score 1 out of max.3). The corresponding irritation categories were I, II and II, respectively. The irritation index (calculated as maximum corneal swelling + maximum mean opacity (x 20) + mean fluorescein score (x 20)) was 40 (out of max. 200). The positive control produced moderate corneal swelling (29%), severe opacity (score 3.0) and severe fluorescein retention (score 3.0). The calculated irritation index was 149. Based on the results of this in vitro study, the substance is considered to be not irritating to the eyes.
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