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EC number: 240-815-0 | CAS number: 16752-77-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EU Commission Directive 87/302/EEC (1988)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Methomyl technical
DPX-X1179-512
Purity: 98.6% - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- deionised water
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20 °C ± 2 °C
- Nominal and measured concentrations:
- Nominal: 0, 6.25, 12.5, 25, 50, 100 and 200 mg/kg
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 23 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- other: body weight
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- other: body weight
- Details on results:
- At all of the tested concentrations of the test substance all earthworms were stiff and inflexible showing little reaction after a mechanical stimulus after 7 and 14 days of exposure. The degree of the observed sublethal effects were the same for all tested concentrations and for both assessment dates (no increase or decrease).
- Results with reference substance (positive control):
- The LC50 for the toxic reference standard was between 10 and 18 mg/kg dry artificial soil; this is within accepted limits (according to experience between 10 and 56 mg/kg dry weight) and confirms the validity of this test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 14-d LC50 = 23 mg/kg dry soil
14-d NOEC = 6.25 mg/kg dry soil (for mortality)
14-d NOEC = 6.25 mg/kg dry soil (for weight loss)
14-d LOEC = 12.5 mg/kg dry soil (for mortality)
14-d LOEC = 12.5 mg/kg dry soil (for weight loss) - Executive summary:
The acute toxicity ofthe test material to earthworms, Eisenia foetida Michaelsen, was estimated in a 14-day soil exposure laboratory study conducted according to OECD Guideline 207 (1984) and EU Commission Directive 87/302/EEC (1988). Four replicates of ten clitellated adult earthworms each were exposed to nominal concentrations of 0, 6.25, 12.5, 25, 50, 100 and 200 mg/kg dry artificial soil (ppm in soil). Controls were replicated four times, with ten earthworms in each replicate. The toxic standard, 2-chloroacetamide, was tested at 0, 10, 18, 32, 56, 100 mg/kg dry artificial soil. Earthworms were assessed for mortality and behavioral effects after 7 and 14 days of exposure and earthworm body weights were assessed at day 0 and day 14.
The LC50 was calculated to be 23 mg/kg dry soil with a lower 95 % confidence limit of 20 and an upper confidence limit of 27 mg/kg dry weight. The NOEC for mortality and weight loss was determined to be 6.25 mg/kg dry soil, the LOEC for mortality and weight loss was determined to be 12.5 mg/kg dry soil. The lowest treatment level causing 100 % mortality was recorded at concentration of 100 mg/kg dry weight soil. Following sub-lethal behavioral effects were observed after 7 and 14 days of exposure. At all of the tested concentrations of the test substance all earthworms were stiff and inflexible showing little reaction after a mechanical stimulus. The degree of the observed sub-lethal effects were the same for all tested concentrations of the test substance and for both assessment dates (no increase or decrease). The LC50 for the toxic reference standard was between 10 and 18 mg/kg dry artificial soil; this is within accepted limits (according to experience between 10 and 56 mg/kg dry weight) and confirms the validity of this test.
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)
- Version / remarks:
- draft (1983)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Recommendations of the Ad Hoc Working Group of the German Federal Biological Institute (1984)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Methomyl technical
DPX-X1179-512
Purity: 98.6% - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- deionized water
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20±2 °C
- pH:
- 6.0±0.5
- Nominal and measured concentrations:
- Nominal: 0, 0.01, 0.1, 1.3, 6.25, 12.5, 25, 50, 100, and 200 mg/kg
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 19 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- other: weight loss
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.25 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.25 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- other: weight loss
- Details on results:
- At the concentration of 1 mg/kg dw, the first sublethal effects were recorded during the study. These effects were significant at concentrations ≥ 6.25 mg/kg dw.
- Results with reference substance (positive control):
- LC50 was between 18 and 32 mg/kg DW; this is within accepted limits and confirms the validity of the test
- Validity criteria fulfilled:
- yes
- Conclusions:
- 14-d LC50 = 19 mg/kg dry soil
14-d NOEC = 3 mg/kg dry soil (for mortality)
14-d NOEC = 3 mg/kg dry soil (for weight loss)
14-d LOEC = 6.25 mg/kg dry soil (for mortality)
14-d LOEC = 6.25 mg/kg dry soil (for weight loss) - Executive summary:
The acute toxicity of the test material to earthworms, Eisenia foetida Michaelsen, was estimated in a 14-day soil exposure laboratory study conducted according to OECD Guideline 207, ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate) and Recommendations of the Ad Hoc Working Group of the German Federal Biological Institute (1984). Controls were replicated four times, with 10 earthworms in each replicate. Nominal concentrations of 0, 0.01, 0.1, 1.3, 6.25, 12.5, 25, 50, 100, and 200 mg/kg dry artificial soil (ppm in soil) were tested. The toxic standard, 2-chloroacetamide, was tested at 0, 10, 18, 32, 56, 100 mg/kg dry artificial soil. Earthworms were assessed for mortality and behavioral effects after 7 and 14 days of exposure and earthworm body weights were assessed at day 0 and day 14.
The LC50 was calculated to be 19 mg/kg dry soil. The NOEC for mortality and weight loss was determined to be 3 mg/kg dry soil, the LOEC for mortality and weight loss was determined to be 6.25 mg/kg dry soil. The lowest treatment level causing 100 % mortality was recorded at concentration of 100 mg/kg dry weight soil. At the concentration of 1 mg/kg dw, the first sub-lethal effects were recorded during the study. These effects were significant at concentrations ≥ 6.25 mg/kg dw. The LC50 for the toxic reference standard was between 10 and 18 mg/kg dry artificial soil; this is within accepted limits (according to experience between 10 and 56 mg/kg dry weight) and confirms the validity of this test.
Referenceopen allclose all
Description of key information
Duration/Value Type | Species | Value | Guideline | Reliability |
14-d LC50 |
Eisenia foetida Michaelsen | 23 mg/kg dry soil | OECD 207, EU Commission Directive 87/302/EEC (1988) | 1 |
14-d NOEC | Eisenia foetida Michaelsen | 6.25 mg/kg dry soil | OECD 207, EU Commission Directive 87/302/EEC (1988) | 1 |
14-d LC50 | Eisenia foetida Michaelsen | 19 mg/kg dry soil | OECD 207, ISO 11268-1 | 1 |
14-d NOEC | Eisenia foetidaMichaelsen | 3 mg/kg dry soil | OECD 207, ISO 11268-1 | 1 |
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 19 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 3 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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