Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-815-0 | CAS number: 16752-77-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- up-and-down procedure
Test material
- Reference substance name:
- Methomyl
- EC Number:
- 240-815-0
- EC Name:
- Methomyl
- Cas Number:
- 16752-77-5
- Molecular formula:
- C5H10N2O2S
- IUPAC Name:
- (E)-[1-(methylsulfanyl)ethylidene]amino N-methylcarbamate
- Test material form:
- solid: crystalline
Constituent 1
- Specific details on test material used for the study:
- Methomyl Technical
Lot number: DPX-X1179-599
Purity: 99.6%
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Ace Animals, Inc., Boyertown, Pennsylvania.
- Females nulliparous and non-pregnant: Yes
- Age at study initiation: Young adult (9-12 weeks)
- Weight at study initiation: 190-232 grams
- Fasting period before study: Yes
- Housing: Animals were singly housed in suspended stainless steel caging with mesh floors. Enrichment (e.g. nylabone) was placed in each cage. Litter paper was placed beneath the cage and was changed at least three times per week.
- Diet: Purina Rodent Chow #5012
- Water: Filtered tap water, ad libitum
- Acclimation period: 8-28 days
ENVIRONMENTAL CONDITIONS
- Temperature: 18-21ºC
- Humidity: 34-65%
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 2.5% w/v mixture in distilled water
- Doses:
- 3.2, 10.1, 32 and 101 mg/kg b.wt.
- No. of animals per sex per dose:
- 3.2 mg/kg: 1
10.1 mg/kg: 3
32 mg/kg: 3
101 mg/kg: 1 - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were observed for mortality, signs of toxicity, and behavioral changes changes within 30 minutes of dosing and during the first several hours post-dosing and at least once daily thereafter for 14 days after dosing of until death occurred. Individual weights of the animals were recorded prior to test substance administration (initial) and again on Days 7 and 14 following dosing or after death.
- Necropsy of survivors performed: Yes
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 17.98 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One animal dosed at 10.1 mg/kg and two dosed at 32 mg/kg died within one hour of test substance administration. The rat dosed at 101 mg/kg died within 30 minutes of test substance administration.
- Clinical signs:
- other: Clinical signs noted in the decedents prior to death of animals dosed at 10.1 mg/kg and 32 mg/kg included hypoactivity, tremors and/or prone posture. The surviving rats from these dose groups exhibited clinical signs including hypoactivity, tremors and/or
- Gross pathology:
- Gross necropsy of the decedent animals dosed at 10.1 mg/kg and 32 mg/kg revealed discoloration of the intestines. No gross abnormalities were noted for any of the euthanized animal dosed at 10.1 mg/kg and 32 mg/kg necropsied at the conclusion of the 14-day observation period. Gross necropsy of the decedent animal at 101 mg/kg revealed discoloration of the intestines.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 based on GHS criteria
- Conclusions:
- Oral LD50 (Rat, female): 17.98 mg/kg
- Executive summary:
An acute oral toxicity test (Up and Down Procedure) was conducted with rats to determine the potential for the test substance to produce toxicity from a single dose via the oral route in accordance with the OECD Guideline 425 and U.S. EPA Guideline OPPTS 870.1100.
Based on an estimated LD50 of 32 mg/kg, a Main Test was conducted using a default starting dose level of 10.1 mg/kg administered to one healthy female rat by oral gavage. Following the Up and Down procedure, two additional females were dosed at 10.1 mg/kg, one female was dosed at 3.2 mg/kg, three females were dosed at 32 mg/kg and one female was dosed at 101 mg/kg. Females were selected for the test because they are frequently more sensitive to the toxicity of test compounds than males. All animals were observed for mortality, signs of gross toxicity, and behavioral changes at least once daily for 14 days after dosing or until death occurred. Body weights were recorded prior to administration and again on Days 7 and 14 (termination) following dosing or after death. Necropsies were performed on all animals.
The animal dosed at 3.2 mg/kg survived and exhibited no clinical signs or body weight loss during the study. No gross abnormalities were noted for this animal when necropsied at the conclusion of the 14-day observation period.
One animal dosed at 10.1 mg/kg and two dosed at 32 mg/kg died within one hour of test substance administration. Clinical signs noted in the decedents prior to death included hypoactivity, tremors and/or prone posture. The surviving rats from these dose groups exhibited clinical signs including hypoactivity, tremors and/or piloerection on the day of dosing. All surviving animals recovered from these signs within one day post-dosing and appeared active and healthy for the remainder of the 14-day observation period, showing no body weight loss during the study. Gross necropsy of the decedents revealed discoloration of the intestines. No gross abnormalities were noted for any of the euthanized animal necropsied at the conclusion of the 14-day observation period.
The rat dosed at 101 mg/kg died within 30 minutes of test substance administration. Clinical signs noted in the decedent prior to death included hypoactivity and prone posture. Gross necropsy of the decedent revealed discoloration of the intestines.
Under the conditions of this study, the acute oral LD50 of the test substance is estimated to be 17.98 mg/kg of body weight (based on maximum likelihood) in female rats with a 95% profile-likelihood based confidence interval of 0 mg/kg (lower) to greater than 20000 mg/kg (upper).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)