Acetamide, 2-chloro-N-[2-(phenylmethyl)phenyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-benzylphenyl)-2-chloroacetamide

EC Number:

606-793-5

Cas Number:

21535-43-3

Molecular formula:

C15 H14 Cl N O

IUPAC Name:

N-(2-benzylphenyl)-2-chloroacetamide

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

other: Milli-RO wtaer

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study, WAL 801 CHLORACETAMID did not induce acute immobilisation of Daphnia magna at 2.9 mg/I after
48 hours of exposure (NOEC).
The 24h-EC50 was 5.2 mg/I with a 95% confidence interval between 4.7 and 6.1 mg/I.
The 48h-EC50 was 4.3 mg/I with a 95% confidence interval between 4.0 and 4.7 mg/I.

Executive summary:

Acute Toxicity Study in Daphnia magna with WAL 801 CHLORACETAMID.

The study procedures described in this report were based on the ISO International Standard 6341: "Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test, Third edition, 1996-04-01.

WAL 801 CHLORACETAMID is a white solid with a purity of 99.37% and not completely soluble at a concentration of 100 mg/I. After a limit study, a final EC50 test was performed with Daphnia exposed for a maximum of 48 hours to a range of concentrations corresponding with 5.6 to 100% of a 5 IJm filtrate prepared at nominal 100 mg/I. The test was performed in duplicate with 10 daphnia per vessel. Sampies for analysis of actual exposure concentrations were taken at the start and the end of the 48-hour test period.

Analysis of the sampies taken during the final EC50 test showed that the measured concentration in the 100 mg/I filtrate corresponded with 9 mg/I and remained stable during the 48-hour test period. Hence, the recovery was 9%. The actual concentrations measured in the sampies taken from the dilutions were in agreement with the expected concentrations based on 9% recovery in the filtrate. The actual concentration range tested was 0.50, 0.90, 1.6, 2.9, 5.0 and 9.0 mg/I.

WAL 801 CHLORACETAMID did not induce acute immobilisation of Daphnia magna at 2.9 mg/I after 48 hours of exposure (NOEC).

The 24h-EC50 was 5.2 mg/I with a 95% confidence interval between 4.7 and 6.1 mg/I.

The 48h-EC50 was 4.3 mg/I with a 95% confidence interval between 4.0 and 4.7 mg/I