Acetamide, 2-chloro-N-[2-(phenylmethyl)phenyl]-

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-benzylphenyl)-2-chloroacetamide

EC Number:

606-793-5

Cas Number:

21535-43-3

Molecular formula:

C15 H14 Cl N O

IUPAC Name:

N-(2-benzylphenyl)-2-chloroacetamide

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA98 and TA100

Additional strain / cell type characteristics:

not specified

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 98 and TA100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Applicant's summary and conclusion

Conclusions:

Based on the results of this study it is concluded that WAL 801 CHLORACETAMID is not mutagenic in the bacterial reverse mutation assay for Salmonella typhimurium TA 100 and TA 98 with and without S9 metabolic activation.

Executive summary:

WAL 801 CHLORACETAMID was tested in the Salmonella typhimurium reverse mutation assay with two histidine-requiring strains of Salmonella typhimurium (TA 100 and T A98).

WAL 801 CHLORACET AMI D was tested up to concentrations of 5000 ~g/plate in the absence and the presence of S9-mix. WAL 801 CHLORACETAMID precipitated on the plates at dose level of 1 000 ~g/plate and upwards.

The bacterial background lawn was not reduced at all concentrations tested in both tester strains. In tester strain T A98, an extreme reduction in the number of revertants was observed at the test substance concentration of 5000 ~g/plate both in the absence and presence of S9-mix. In tester strain TA 100, an extreme reduction in the number of revertants was observed at test substance concentrations of 3330 and 5000 ~g/plate both in the absence and presence of S9-mix.

WAL 801 CHLORACETAMID did not induce a dose-related increase in the number of revertant colonies both in the absence and the presence of S9-mix.

Based on the results of this study it is concluded that WAL 801 CHLORACETAMID is not mutagenic in the Salmonella typhimurium reverse mutation assay.