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EC number: 202-393-6 | CAS number: 95-13-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 October 2007 - 21 November 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 17 July 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Indene
- EC Number:
- 202-393-6
- EC Name:
- Indene
- Cas Number:
- 95-13-6
- Molecular formula:
- C9H8
- IUPAC Name:
- indene
- Test material form:
- not specified
- Details on test material:
- - Details on test material are confidential
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- - Source of inoculum/activated sludge: sludge was collected from 10 different locations in Japan (4 sewage treatment plants, 3 rivers, 1 lake and 2 bays) in September 2007.
- Method of collection from sewage plant: return sludge was collected. Method of collection from rivers, lake and sea: surface water and surface soil which was in contact with the atmosphere were collected.
- Method of cultivation: After ceasing aeration for approx. 30 minutes, supernatant of the sludge mixture corresponding to about 1/3 of the whole colume was removed. Dechlorinated water was added to the mixture so that the concentration of the synthetic sewage was 0.1% in the volume of dechlorinated water added. This procedure was repeated once every day at a temperature of 25±2°C
- Preparation of inoculum for exposure: the filtrate (5 L) of the supernatant of the activated sludge cultivated for 3 months was mixed with the mixed filtrate (5 L) of the supernatant of a sludge collected newly at each location. The mixed filtrate (10 L) was aerated with prefiltered open air after the pH value was adjusted to 7.0 +/- 1.0.
- Concentration of sludge: 3970 mg suspended solids/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- (HPLC)
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301C
- Additional substrate: no
- Test temperature: 25±1°C
- pH: not measured due to volatility of the test item
- pH adjusted: no, only the pH of the initial inoculum was adjusted
- Aeration of dilution water: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
- Other: each test solution was stirred with a magnetic stirrer
TEST SYSTEM
- Culturing apparatus: closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: 3 bottles for the test item + sludge, 1 bottle for the other vessels (see below)
- Measuring equipment: automatic data sampler
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency and method: BOD was measured automatically with a data sampler, HPLC was used at the end of the test to analyse the amount of test item left in the system.
- Other: appearance of the test solution was observed once a day
CONTROL AND BLANK SYSTEM
- Blank control: yes, mineral medium only (1 bottle)
- Abiotic control: yes, test item in water (1 bottle)
- Toxicity control: no
- Other: reference control (1 bottle, reference item in medium)
Reference substance
- Reference substance:
- aniline
- Remarks:
- (100 mg/L)
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: regarded as 0% because the calculated average value was calculated to be negative
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- - Degradation percentages in individual bottles were -6, -6 and -4% (based on BOD) and 0, 1 and 1% (based on HPLC).
BOD5 / COD results
- Results with reference substance:
- Aniline was degraded for 75% after 14 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'overall remarks'
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on a biodegradation study (MITI test), performed according to OECD guideline 301 C and GLP principles, no biodegradation of Indene was observed after a 28 day test duration.
- Executive summary:
In a MITI test, performed according to OECD guideline 301 C and GLP principles, Indene was assessed for its biodegradation over a period of 28 days. Sludge was collected from ten different sites and inoculated for a period of 3 months. This inoculum was used to prepare the mineral medium for the study. The test item was tested in mineral medium containing sludge at a concentration of 100 mg/L in triplicate next to one replicate of test item in water, the reference item (aniline) in mineral medium and one control blank containing only mineral medium. During the test the amount of consumed oxygen was measured and at the end of the test period the amount of remaining test item was analysed by using HPLC. Based on the BOD the average % degradation was calculated to be 0% for the 3 replicates. Based on HPLC analysis, the average % degradation was calculated to be 1% for the 3 replicates. The acceptability criteria were met and the study was considered to be valid. Based on the results, Indene is considered to be not readily biodegradable.
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