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EC number: 204-427-5 | CAS number: 120-80-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- mechanistic studies
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Test well described, but the test substance purity is not reported.
Data source
Reference
- Reference Type:
- publication
- Title:
- No information
- Author:
- Wilmer J. L. et al.
- Year:
- 1 997
- Bibliographic source:
- In Vitro Toxicology, 10(4), 429-436.
Materials and methods
- Principles of method if other than guideline:
- In vitro effects on proinflammatory cytokines and growth factors in human epidermal keratinocytes.
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Pyrocatechol
- EC Number:
- 204-427-5
- EC Name:
- Pyrocatechol
- Cas Number:
- 120-80-9
- Molecular formula:
- C6H6O2
- IUPAC Name:
- pyrocatechol
- Details on test material:
- Purchased from Sigma, purity unknown.
Constituent 1
Test animals
- Species:
- human
- Strain:
- other: epidermal keratinocytes
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- - Test system: Cryopreserved normal human keratinocytes (NHK) from breast skin of adult females were used.
Administration / exposure
- Route of administration:
- other: in vitro
- Duration of treatment / exposure:
- Exposure period: 4 hour(s)
Doses / concentrations
- Remarks:
- Doses / Concentrations:
11, 22, 44 µg/mL (100, 200 and 400 µM)
Basis:
nominal conc.
- No. of animals per sex per dose:
- Not applicable
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Cell culture and chemical treatment:
Keratinocytes were cultured in Keratinocyte Growth Medium. The cells were trypsinized and subcultured in wells at a seeding density of 2.4 x 10E4 cells/cm2. After 24 h, at which time the cells were approximately 70% confluent, the complete medium was changed to growth medium without hydrocortisone, bovine pituitary extract and rh epidermal growth factor.
Catechol was dissolved in phosphate buffered saline and sterile-filtered. Exposure of the cultures to catechol (11, 22, 44 mg/L), delivered in 10 mL aliquots, was for 4 hours in a humidified 5% CO2/95% air atmosphere. After exposure, the cultures were rinsed, and fresh growth factor-deficient medium in 4 mL aliquots was pipetted into each flask for a further 20-h culture period. The culture supernatants were removed, sterile-filtered into cryotubes and frozen at -70°C. Keratinocyte viability was assessed concurrently using a modified in situ trypan blue dye exclusion procedure. Four to six cultures were tested per experiment.
- Cytokine measurements:
* For IL-8, wells were coated with capture antibodies (mouse antihuman IL-8 monoclonal antibody) at a concentration of 2 µg/mL of coating buffer for 24 h at 4°C. After having blocked the free spaces, samples or standards were added in 0.1 mL aliquots and incubated for 2 h at 37°C. 0.1 mL of goat antihuman IL-8 polyclonal IgG antibody, diluted to 0.5 µg/mL, was added to each well for an additional 2 hours. The plates were incubated with peroxidase-conjugated rabbit antigoat IgG antibody, diluted 1:7500, for 1 hour. The cells were then incubated with peroxidase substrate for 20 minutes and the reaction was terminated by the addition of 50 µL of 2N sulfuric acid. Between each protocol phases, cells were washed with PBS.
* TGF-alpha concentrations were determined using a commercial enzyme-linked immunosorbent assay (ELISA). Absorbency was read at 450 nm using an UV computer reader.
- Statistical analysis:
The concentration-response was analysed initially by Bartlett's test for homogeneity of variances, and in some cases the data were transformed by the square-root to equalise variances prior to one-way analysis of variance (ANOVA). If the F-statistic was significant, post hoc comparisons were made using Tukey's honestly significant differences procedure to determine whether the individual treatment groups were significantly different (p < 0.05).
Results and discussion
- Details on results:
- * Catechol was not cytotoxic in NHK cells at the concentrations tested as assessed by trypan blue.
* Catechol did not induce TGF-alpha and had slight suppressive effects on both constitutive TGF-alpha and IL-8 secretions.
- The secretion of TGF-alpha was 42 ± 1 pg/ml at the concentration of 200 µM catechol (not statistically different from the vehicle value: 48 ± 5 pg/ml) and 38 ± 5 pg/ml at the concentration of 400 µM (p < 0.05). Catechol was not tested at the concentration of 100 µM for TGF-alpha secretion.
- The secretion of IL-8 was 1 ± 1 at the concentration of 100 and 200 µM catechol (p < 0.05, when compared to the vehicle value: 4 ± 1 pg/ml) and 0 ± 0 pg/ml at the concentration of 400 µM (p < 0.05).
Applicant's summary and conclusion
- Conclusions:
- Negative
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