Plastic, waste, pyrolized, fractionation bottoms

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

Name TACOIL
Batch no. 19085.09
Appearance clear, amber-brown liquid
Composition see others
CAS No. not assigned
EC-No. 942-177-5
Molecular formula not stated
Molecular weight c. a. 150.5 g/mol
Purity 100 % UVCB
Homogeneity homogeneous
Vapour pressure not stated
Stability H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
Solubility H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
Expiry date 31. Dec. 2025
Storage Room Temperature (20 ± 5 °C)


Preparation
The water-accommodated fractions (WAFs) were prepared for the test. This was done by pipetting the real loads of 46 / 100 / 220 / 460 / 1000 mg/L resp. 59.0 / 128.2 / 282.1 / 589.7 / 1282.1 µL/L test item (based on a density of 0.78 g/mL given by the SDS) of the corre-sponding amount of dilution water and stirring vigorously for 24 ± 1 hours. The resulting solutions were used unfiltrated.

Vehicle:

no

Details on test solutions:

Dilution water (Daphnia medium) with the following specification was used (stated in SOP
Parameter Concentration in mg/L
CaCl2*2H2O 293.80
MgSO4*7H2O 123.30
NaHCO3 64.80
KCl 5.80

Test organisms (species):

Daphnia magna

Details on test organisms:

Young daphnia, aged less than 24 hours
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Acclimatation
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20  2 °C

Test type:

static

Water media type:

brackish water

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Immobilisation was recorded at 24 hours and 48 hours

Test temperature:

18.9 – 20.5 °C

pH:

7.5-7.7

Dissolved oxygen:

7.5-8.4 mg/L

Nominal and measured concentrations:

46 / 100 / 220 / 460 / 1000 mg/L (nominal concentration)

Details on test conditions:

Treatments
46 / 100 / 220 / 460 / 1000 mg/L (nominal concentration)
Temperature 18.9 – 20.5 °C
Duration: 48 hours
Observation times: 24 and 48 hours
Medium renewal: none
Test vessel: Closed glass flasks nominal volume 65 mL
Treatments 4 vessels, each completely filled with test solution and 5 daphnia
Blank control 4 vessels, each completely filled with dilution water and 5 daphnia

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 6.2 - < 9.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 5.41 - < 7.81 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 3.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

The study was performed using 5 concentrations ranging from 46 to 1000 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the im-mobilised daphnia were counted.
Four concentrations showed toxicity between 15 and 100 % immobilisation. None of the animals was immobilised in the blank control.

Validity criteria fulfilled:

yes

Conclusions:

48h-NOEC = 1.50 mg/L
48h-LOEC = 3.90 mg/L
24h-EC50 = 7.58 mg/L
48h-EC50 = 6.50 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 6.5 mg/L