Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-109-3 | CAS number: 103-41-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 972
- Report date:
- 1972
Materials and methods
- Principles of method if other than guideline:
- Groups of test animals (10 per dose group) received the test substance dissolved in corn oil (500 mg/mL) via oral gavage.
Doses ranged from 2.0 to 5.0 g per kg bw. Animals were observed for 14 days post treatment. - GLP compliance:
- no
- Remarks:
- test carried out pre-GLP-guideline
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Benzyl cinnamate
- EC Number:
- 203-109-3
- EC Name:
- Benzyl cinnamate
- Cas Number:
- 103-41-3
- Molecular formula:
- C16H14O2
- IUPAC Name:
- benzyl 3-phenylacrylate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Charles River
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 200-300 g
- Fasting period before study: 48 hours
- Housing: wire mesh cages in air conditioned room
- Diet: regular diet of Lab Blox; ad libitum
- Water: ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- Concentration: 500 mg/mL (w/v)
- Doses:
- 2.0, 2.25, 3.0 and 5.0 g/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, food consumption - Statistics:
- Oral LD50 was calculated according to the method of Litchfield, V.T. and Wilcoxon, F., Journal of Pharmacology and Experimetnal Therapeutics, Vol 96, p 99, 1949.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 280 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2 620 - <= 4 100
- Mortality:
- 8 animals dosed with 5 g/kg bw died within 24 hours post-dosing, the remaining 2 showed a normal increase in body weight at the end of the 14 days observation period.
- Clinical signs:
- Animals dosed with 5 g/kg bw showed CNS effects 18 hours post-dosing.
Animals dosed at the three lower levels did not show any symptoms prior to death and behaved as normal laboratory animals. - Body weight:
- Normal body weight increase.
All surviving animals ate well.
Any other information on results incl. tables
Results:
Doses (g/kg bw) |
Response (deaths) |
% observed |
% expected |
Observed minus expected |
(CHI)2 |
2.0 |
2/10 |
20 |
17 |
3 |
.006 |
2.25 |
2/10 |
20 |
23 |
3 |
.005 |
3.0 |
4/10 |
40 |
43 |
3 |
.004 |
5.0 |
8/10 |
80 |
79 |
1 |
.015 |
N1 = 40
ED84 = 5.48
ED50 = 3.28
ED16 = 1.96
S= [(ED84/ED50) + (ED50/ED16)] / 2 = 1.67
fED50 = S2.77 / √ N1= 1.25
ED50 * fED50 = 4.1
ED50 / fED50 = 2.62
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The oral LD50 was calculated to be 3.28 g/kg bw with 19/20 confidence limit of 2.62 to 4.1 g/kg bw.
- Executive summary:
In the current study the oral LD50 of the test substance was determined in rats in an oral (gavage) acute toxicity test.
No OECD guideline was followed and the study was not GLP.
The rats were fed 2.0, 2.25, 3.0 or 5.0 g/kg bw of the test material dissolved in corn oil, via a rigid stomach tube. 10 animals per dose were used. Following the administration of each dose level the animals were observed for 14 days for signs of toxicity.
8/10 animals dosed with 5 g/kg bw died within 24 hours post-dosing, the remaining 2 showed a normal increase in body weight at the end of the 14 days observation period. All surviving animals ate well and gained body weight in a normal way.
Animals dosed with 5 g/kg bw showed CNS effects 18 hours post-dosing. Animals dosed at the three lower levels did not show any symptoms prior to death and behaved as normal laboratory animals.
The oral LD50 was calculated to be 3.28 g/kg bw with 19/20 confidence limit of 2.62 to 4.1 g/kg bw.
As the LD50 > 2,000 mg/kg bw, the test item is not to be classified as acute toxic according to the CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.