[29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]iron

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Short term toxicity of test chemical to aquatic algae was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Remarks:

DMSO

Details on test solutions:

The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Remarks on exposure duration:

±1 hour

Test temperature:

23±2°C

pH:

Test at highest concentration: 7.9 (Change to 7.7 during tests)
Control: 8.2 (changed to 8 during test)
Control + DMSO: 8 (changed to 7.7 during test)

Nominal and measured concentrations:

Limit test performed at 21 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

72 h

Dose descriptor:

other: IC

Remarks:

%

Effect conc.:

21 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 1.6 % inhibition were observed at 21 mg/l.

Results with reference substance (positive control):

Results with reference substance valid
- EC50: 0.75 mg/L

Reported statistics and error estimates:

The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05.

Validity criteria fulfilled:

yes

Conclusions:

Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of test chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration.

Executive summary:

Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.

Description of key information

Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.

Key value for chemical safety assessment

EC50 for freshwater algae:

21 mg/L

Additional information

Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.