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EC number: 240-505-5 | CAS number: 16455-61-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-11-20 - 2010-01-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No surrogate or analogue material was used. - Analytical monitoring:
- not required
- Details on sampling:
- Not applicable.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Test substance: 2.0 g of the test item was added to about 800 mL of demineralized water and stirred at room temperature until the test item was completely dissolved. After that the pH-value was measured (measured value = 8.4) and adjusted to 7.1 with 1 M sulfuric acid solution. The stock solution was made up to 1 L with demineralized water. The stock solution appeared red brown and dissolved.
- Reference item: 0.5 g of the reference item was added to about 800 mL of demineralized water and stirred at room temperature until the reference item was completely dissolved. The pH value of the stock solution was measured and adjusted to 7.3 with 1 M sodium hydroxide solution. Following this the stock solution was made up to 1 L with demineralized water. The stock solution appeared dissolved and yellowish. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed with tap water one time. After that the suspension was adjusted to 7.5 g/L dry matter.
- Initial biomass concentration: An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.
- Source: municipal wastewater treatment plant of Mannheim, Germany; from the aeration tank of the plant - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period described.
- Hardness:
- Not applicable.
- Test temperature:
- 20 +/- 2 °C
- pH:
- 7.15
- Dissolved oxygen:
- No details available.
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test substance: 1000, 500, 250, 125 and 62.5 mg/L (nominal)
Reference substance: 1, 10 and 100 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer vessel (nominal volume 250 mL)
- Aeration: yes
- No. of vessels per concentration (replicates): 1 (5 concentrations)
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 2
TEST PROCEDURE:
Aliquots of the stock solutions with test- or reference item were pipetted into test vessels and made up with demineralized water to a volume of 150 mL. After that 8 mL synthetic medium were given to the test vessel. To prepare the blank control assays, 150 mL of demineralized water and 8 mL synthetic medium were given to the blank control test vessels. The pH-values were measured in all test vessels. An adjustment was not necessary. 42 mL demineralized water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blank control assays were prepared according to the same procedure without addition of test- or reference item. After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell. A chart recorder recorded the oxygen respiration rates. The oxygen consumption of the blank control BC1 was measured first. The oxygen consumption of the reference item assays (RS) and the test item assays was measured in the sequence RS1, RS2, RS3; TS1, TS2, TS3, TS4 and TS5.
The oxygen consumption of the blank control BC2 was measured at last. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 450 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No further details are available.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: up to 80 % inhibition occurred - Reported statistics and error estimates:
- In order to calculate the inhibition effects of the test item at a particular concentration, the respiration rate was expressed as percentage of the mean of the 2 control respiration rates.
The degree of inhibition (EC10 and EC20) of the test item was evaluated by Probit analysis according to Finney. The values were given with an accuracy of 2 significant digits.
The degree of inhibition (EC20; EC50 and EC80) of the reference item was also evaluated by Probit analysis according to Finney. - Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria of the applied guideline were fulfilled.
- Conclusions:
- The study is regarded as a valid guideline study conducted under certificated GLP compliance. According to the reported EC50 value (> 1000 mg/L, nominal) the test substance is not acutely toxic towards aquatic microorganisms.
- Executive summary:
The toxicity of Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts (CAS 84539-55-9) towards freshwater microorganisms was investigated in accordance to OECD Guideline 209 / EU Method C11 (BASF SE, 2009). Activated sludge was collected from the aeration tank at a municipal wastewater treatment. Aliquots of the stock solutions with test- or reference substance were pipetted into test vessels and made up with demineralized water to a volume of 150 mL. After that 8 mL synthetic medium were given to the test vessel. To prepare the blank control assays 150 mL of demineralized water and 8 mL synthetic medium were given to the blank control test vessels. The pH-values were measured in all test vessels. An adjustment was not necessary. 42 mL demineralized water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blank control assays were prepared according to the same procedure without addition of test- or reference item. After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell. As results, an EC50(3h) and EC80(3h) > 1000 mg/L and an EC10(3h): 450 mg/L is reported.
Reference
Measured data of oxygen content of the test assays at the begin and end of the evaluation time:
Assay identification |
BC1 |
BC2 |
RS1 |
RS2 |
RS3 |
TS1 |
TS2 |
TS3 |
TS4 |
TS5 |
O2-concencentration Start [mg/L] |
8.0 |
8.0 |
8.4 |
8.7 |
9.1 |
8.2 |
8.3 |
8.5 |
7.8 |
7.6 |
O2 -concentration after 6 minutes [mg/L] |
7.0 |
7.0 |
7.5 |
8.2 |
8.9 |
7.5 |
7.5 |
7.5 |
6.5 |
6.0 |
O2consumption rate [mg/L in 6 min] |
1.0 |
1.0 |
0.9 |
0.5 |
0.2 |
0.7 |
0.8 |
1.0 |
1.3 |
1.6 |
O2-consumption rate [mg/Lxh] |
10 |
10 |
9 |
5 |
2 |
7 |
8 |
10 |
13 |
16 |
Specific O2-consumption rate [mg O2/gxh] |
7 |
7 |
6 |
3 |
1 |
5 |
5 |
7 |
9 |
11 |
RS/TSconcentrations[mg/L] |
- |
1 |
10 |
100 |
1000 |
500 |
250 |
125 |
62.4 |
|
Calculation of inhibition respiration[%] |
- |
- |
10 |
50 |
80 |
30 |
20 |
0 |
-30 |
-60 |
Legend: TS = test item, RS = reference item, BC = blank control
Description of key information
EC10 (3h): 450 mg/L, EC50 (3h) > 1000 mg/L [OECD 209; activated sludge; read-across substance: Fe(Na)EDDHA]
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 450 mg/L
Additional information
Toxicological effects towards aquatic microorganisms of o-o-EDDHA (CAS16455-61-1) was not investigated experimentally. Read across to the structurally similar substance Fe(Na)EDDHA (CAS 84539-55-9) was performed to fulfill this relevant endpoint under REACH (for further details, please refer to the separate read-across statement).
The toxicity of Fe(Na)EDDHA (CAS 84539-55-9) towards freshwater microorganisms was investigated in accordance to OECD Guideline 209 / EU Method C11 (BASF SE, 2009). Activated sludge was collected from the aeration tank at a municipal waste water treatment. Aliquots of the stock solutions with test- or reference substance were pipetted into test vessels and made up with demineralized water to a volume of 150 mL. After that 8 mL synthetic medium were given to the test vessel. To prepare the blank control assays 150 mL of demineralized water and 8 mL synthetic medium were given to the blank control test vessels. The pH-values were measured in all test vessels. An adjustment was not necessary. 42 mL demineralized water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blank control assays were prepared according to the same procedure without addition of test- or reference item. After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell. As results, an EC50(3h) and EC80(3h) > 1000 mg/L and an EC10(3h): 450 mg/L is reported.
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