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EC number: 294-436-0 | CAS number: 91722-33-7 A complex combination of organic compounds separated after condensation of the vapors from the destructive distillation of wood.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
The acute oral toxicity of Tarwood was investigated in a study in rats that was performed in accordance with the requirements of the following guidelines and test methods:
- OECD GUIDELINES FOR TESTING OF CHEMICALS 423 (17th December 2001)
- Council Regulation (EC) No 440/2008 (30 May 2008),
- Regulation (EC) No. 1272/2008
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21.01.2014 - 06.02.2014
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Crl:(WI)BR rats
- Sex:
- female
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Doses:
- 2000 mg/kg bw
- Control animals:
- not specified
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Clinical signs:
- other:
- Interpretation of results:
- not classified
- Remarks:
- Migrated informationCriteria used for interpretation of results: EU
- Conclusions:
- The method used, was not intended for the precise calculation of a precise LD50 value. The test item was ranked into classes of Globally Harmonized Classification System (GHS) described in the OECD Guideline No. 423.
- Executive summary:
An acute toxic class method was carried out involving a stepwise procedure with the use of 2000 mg/kg bw as the starting dose in three female rats. No animal died in the first step at 2000 mg/kg bw dose level, therefore treatment with 2000 mg/kg bw was repeated on further three female rats. No animal died in the second step, too, so the test was finished, the stopping criteria of Annex 2d of OECD Guideline No. 423 (presented in Appendix VII) was met. Animals were weighed, observed for lethality and toxic symptoms for 14 days after the treatment. Gross pathological examination was carried out on the 15th day after the treatment.
Reference
see study report for further information
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 000 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 August 2019 - 20 September 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 433 draft (Acute Inhalation Toxicity: Fixed Concentration Procedure) (not officially approved)
- Version / remarks:
- 2018
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- fixed concentration procedure
- Limit test:
- no
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Raleigh, North Carolina 27610
- Age at study initiation: Approximately 9 weeks
- Weight at study initiation: 249.7 g to 265.2 g
- Fasting period before study: not specified
- Housing: Teklad 7070C Certified Diamond Dry Cellulose Bedding, Envigo, Madison, Wisconsin; 2-3 animals per cage; entichment provided
- Diet ad libitum: Teklad Global 16% Protein Rodent Diet (Certified), 2016C, Envigo, Madison, Wisconsin
- Water ad libitum: pot water
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 to 26 °C
- Humidity (%): 30 to 70%.
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: To:
AM Morning observation
PM Afternoon observation
RTC Observations upon return to their home cage after exposure
1HrPD Observations 1 hour after exposure
2HrPD Observations 2 hours after exposure
NDD Observations on non-exposure days - Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Remarks:
- liquid aerosol
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- ca. 2.2 µm
- Geometric standard deviation (GSD):
- >= 1.78 - <= 1.81
- Remark on MMAD/GSD:
- MMAAD Gravimetr.: 2.3 (std: 1.77 - 1.82)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:
- Exposure chamber volume:
- Method of holding animals in test chamber:
- Source and rate of air:
- Method of conditioning air:
- System of generating particulates/aerosols:
- Method of particle size determination:
- Treatment of exhaust air:
- Temperature, humidity, pressure in air chamber: 20°C, 56% rel hum.
TEST ATMOSPHERE
- Brief description of analytical method used: HPLC/UV
- Samples taken from breathing zone: yes
VEHICLE
- Composition of vehicle (if applicable): not applicble
- Concentration of test material in vehicle (if applicable): not applicble
- Justification of choice of vehicle: not applicble
- Lot/batch no. (if required): not applicble
- Purity: not applicble
TEST ATMOSPHERE (if not tabulated)
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: Wood Tar has been evaluated for acute oral toxicity in rats and was found to be non-lethal at 2000 mg/kg. Also, an in vitro evaluation of dermal toxicity was done using human skin and the test item was found to be irritating but not corrosive. On the basis of these results which indicate that the test might have low toxicity, the initial exposure level for this study was selected to be 1.0 mg/L as per the OECD Guidelines (OECD 433, 2018). - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- target: 1,0 mg/L
analytical: 1,6 mg/L - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Key result
- Sex:
- male
- Dose descriptor:
- LC0
- Effect level:
- 1.6 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 1.6 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- not determinable because of methodological limitations
- Mortality:
- Not occured
- Clinical signs:
- other: not occured
- Body weight:
- unchanged
- Gross pathology:
- No macropathology findings, in one animal testes small bilateral, soft bilateral. Not considered to be an effect of treatment.
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- The presented GLP-inhalation study followed international guidance and no deviations from guideline were reported.
The single reported effect concentration is the starting concentration of a planned series of concentrations, and represents the only achievable concentration of the test item. Therefore it is concluded the reported value represents the NOEC and the LOEC and LOEC and the LC50 must be >1.6 mg/L. - Executive summary:
Sprague-Dawley CD® rats (5 males) were exposed once to Wood Tar by nose-only inhalation of liquid aerosol at 1.0 mg/L of air for 4 hours. After a 14-day observation period the animals were euthanized and necropsied. Parameters evaluated during the study were: viability, clinical observations, body weights and macroscopic observations.
Wood Tar was exposed to rats for 4 hours at 1.6 mg/L based on analytical results. This exposure level was well tolerated and a higher exposure level of 5.0 mg/L was thus planned. However, additional aerosol trials demonstrated that the exposure at the target of 1.0 mg/L was essentially a maximum attainable exposure level. Therefore, the 5.0 mg/L exposure was not conducted and the study was considered completed.
Thus, the LC50 would be estimated to be >1.6 mg/L.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 1 600 mg/m³ air
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
An oral acute toxic class method was carried out involving a stepwise procedure with the use of 2000 mg/kg bw as the starting dose in three female rats. No animal died in the first step at 2000 mg/kg bw dose level, therefore treatment with 2000 mg/kg bw was repeated on further three female rats. No animal died in the second step, too, so the test was finished, the stopping criteria of Annex 2d of OECD Guideline No. 423 (presented in Appendix VII) was met. Animals were weighed, observed for lethality and toxic symptoms for 14 days after the treatment. Gross pathological examination was carried out on the 15th day after the treatment.
A second study following the inhalation expsoure route was conducted with the only achived test concentration of 1.5 mg/L and did not revealed any effects.
Lethality, Clinical symptoms and Body weight:
No lethality was noted at single oral dose of 2000 mg/kg bw. No clinical symptoms were observed on the day of the treatment and during the 14-day observation period, the general state and behaviour of experimental animals were normal. The body weight development was undisturbed in all animals.
Gross pathology:
All animals survived until the scheduled autopsy on Day 15. All organs of all experimental animals proved to be free of treatment related gross pathological changes.
Evaluation:
The method used is not intended to allow the calculation of a precise LD50 value. The test item was ranked into classes of Globally Harmonized Classification System (GHS) described in the OECD Guideline No. 423 as below:
Dose (mg/kg bw) |
Mortality (dead/treated) |
LD50(mg/kg bw) |
GHS category |
2000 |
0/6 |
above 2000 |
5 |
Justification for selection of acute toxicity – inhalation
endpoint
The inhalation toxicity of Tarwood has been tested on Sprague-Dewley
rats following OECD TG 433 in a valid GLP study. At the highest
technical achievable concentration of 1.6 mg/L over 4 hours no toxic
effects or signs of clinical symptoms have been observed. Therefore is
it concluded 1.6 mg/L < LC50 inhalation in rats.
Justification for classification or non-classification
Following OECD GUIDELINE FOR TESTING OF CHEMICALS No 423 Annex 2d no mortality was observed.
No death occurred at 2000 mg/kg bw single oral dose of Tar wood. All female rats in step 1 and step 2 survived.
The acute inhalation test following OECD TRG 433 with 1.6 mg/L over a period of 4 hours in rats did not led to any symptoms, all animals survived.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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