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EC number: 266-442-3 | CAS number: 66669-53-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/93
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- , the light intensity was slightly too high (25 instead of max. 20 µE/m2*s), CSB was determined.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- Not indicated
- Test temperature:
- ca. 20°C +/-2°C; per single test: 20°C +/-1°C
- pH:
- Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6 - Dissolved oxygen:
- Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1 - Salinity:
- Not indicated
- Nominal and measured concentrations:
- Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159 - Details on test conditions:
- TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus" - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 071 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 329 - < 1 071 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 104 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: DUNNETT-TEST
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 329 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: DUNNETT-TEST
- Details on results:
- The results are expressed in terms of the arithmetic mean of measured concentrations. Effective concentrations ranged from 98.1 to 115.9 % of nominal values during the experiment.
- Reported statistics and error estimates:
- The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L - Executive summary:
A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acidon the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 11/93
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Please find the Justification attached to Chapter 13.2.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- , the light intensity was slightly too high (25 instead of max. 20 µE/m2*s), CSB was determined.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- Not indicated
- Test temperature:
- ca. 20°C +/-2°C; per single test: 20°C +/-1°C
- pH:
- Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6 - Dissolved oxygen:
- Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1 - Salinity:
- Not indicated
- Nominal and measured concentrations:
- Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159 - Details on test conditions:
- TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus" - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 071 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 329 - < 1 071 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 104 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: DUNNETT-TEST
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 329 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: DUNNETT-TEST
- Details on results:
- The results are expressed in terms of the arithmetic mean of measured concentrations. Effective concentrations ranged from 98.1 to 115.9 % of nominal values during the experiment.
- Reported statistics and error estimates:
- The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L - Executive summary:
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.
In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.
Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.
A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acidon the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).
Referenceopen allclose all
Description of key information
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate, results of the parent acid, 2-phosphonobutane-1,2,4-tricarboxylic acid are taken into account for this endpoint.
Prolonged toxicity with Daphnia magna was performed according to "Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991 covering OECD 211) and the following results were obtained:
EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Remarks:
- reproduction
- Effect concentration:
- 104 mg/L
Additional information
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.
In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.
Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.
A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acid on the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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