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EC number: 266-442-3 | CAS number: 66669-53-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Toxicological Summary
- Administrative data
- Workers - Hazard via inhalation route
- Workers - Hazard via dermal route
- Workers - Hazard for the eyes
- Additional information - workers
- General Population - Hazard via inhalation route
- General Population - Hazard via dermal route
- General Population - Hazard via oral route
- General Population - Hazard for the eyes
- Additional information - General Population
Administrative data
Workers - Hazard via inhalation route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- By inhalation
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Workers - Hazard via dermal route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Dermal
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
Workers - Hazard for the eyes
Local effects
- Hazard assessment conclusion:
- no hazard identified
Additional information - workers
Tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (CAS 66669-53-2)
Hazard conclusion systemic (worker/general population) long-term
Basis for hazard conclusion systemic effects (worker/General population):
A repeated dose toxicity study in Wistar rats (90 day feeding study) according OECD Guideline 408 is available.
There is no long-term study available using the inhalation or dermal route. Thus, as starting point for the calculation/assessment of hazard, the NOAEL of the sub-chronic feeding study has to be taken into account.
Study
Title: PBS-AM Subchronische toxikologische Untersuchungen an Ratten (Fütterungsversuch über 3 Monate)
Administration period: 90 days
Doses: 50, 200, 1000, 5000 ppm = male rats: 0, 4.17, 14.91, 84.11, 424.41 mg/kg bw/day, female rats: 0, 6.05, 12.51, 125.48, 632.65 mg/kg bw/day
NOAEL, Effects
NOAEL (systemic) = 424 mg/kg bw (male and female rats)
Effects: All doses were tolerated without any effects
A 3-months feeding study was performed in 15 female and 15 male rats with technical tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (50, 200, 1000, 5000 ppm = male rats: 0, 4.17, 14.91, 84.11, 424.41 mg/kg bw/day, female rats: 0, 6.05, 12.51, 125.48, 632.65 mg/kg bw/day). The control animals (30 females, 30 males) received no test substance (0 ppm).
All doses were tolerated without any effects as could be shown by overall observations and examinations: Appearance, behaviour, development, and mortality as well as blood, blood glucose and cholesterol, metabolism of electrolytes (NA, K, Ca), Ferrum and Phosphorus were not affected by doses up to 5000 ppm. The same was proven for the kidneys by urinalyses, clinical chemistry and pathological and histopathological examinations. Gross necropsy and histological examinations did not reveal any adverse effects due to the test substance.
Thus, the NOAEL is equal or higher than 5000 ppm (equivalent to about 424 mg/kg bw for male rats and 632 mg/kg bw for female rats).
Additional, a developmental/teratogenicity study with 2-phosphonobutane-1,2,4-tricarboxylic acid (CAS 37971-36-1) in Wistar rats equivalent or similar to OECD Guideline 414 (Prenatal Developmental Toxicity Study) is available which is used for read-across.
Study Title: Untersuchung auf embryotoxische Wirkungen an Ratten nach oraler Verabreichung [Examination of embryotoxicity effects in rats following oral administration of Bayhibit-AM]
Administration period: 10 days
Doses: 0, 100, 300 or 1000 mg/kg bw/day
NOEL, Effects
NOEL (systemic) = 1000 mg/kg bw
Effects: No maternal toxicity, no teratogenicity, no embryotoxicity was observed under the study conditions.
After oral application of 2-phosphonobutane-1,2,4 -tricarboxylic acid, (CAS 37971-36-1, a structural analogue, please refer to the read across justification document at IUCLID Chapter 13.2) up to a maximal dosage of 1000 mg/kg, no signs of maternal toxicity were found (by means of death, weight loss, changes in appearance and behaviour). No influence was observed in embryo and foetus development (resorption, placenta weight, any skeletal and internal malformation). The NOEL value for these effects is therefore determined as 1000 mg/kg bw/day.
Under the experimental conditions, the test item is considered to have no maternal and embryonic toxic effects and no teratogenic effects in rats.
Thus, the NOAEL is equal or higher than 1000 mg/kg bw.
Conclusion: In the sub-chronic study and the developmental/teratogenicity study no adverse effects were observed. Therefore ‘no hazard’ is identified for systemic effects.
Hazard conclusion systemic (worker/general population) short-term
Tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (CAS 66669-53-2) is not classified for acute oral, acute dermal and acute inhalation toxicity.
Conclusion: Therefore ‘no hazard’ is identified for systemic effects (short term).
Hazard conclusion local effects (worker/general population)
Basis for hazard conclusion local (long- and short-term toxicity):
Irritation/corrosion
In the key studies for skin irritation/corrosion and eye irritation, the test substance (tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate) is not irritating to the skin and eyes.
A classification is not justified.
Sensitization
In a Guinea Pig maximization test (GPMT) the test substance (tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate) revealed no sensitisation potential.
A classification is therefore not justified.
Conclusion: For local effects no hazard is identified.
General Population - Hazard via inhalation route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- By inhalation
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
General Population - Hazard via dermal route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Dermal
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
General Population - Hazard via oral route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
- Route of original study:
- Oral
DNEL related information
General Population - Hazard for the eyes
Local effects
- Hazard assessment conclusion:
- no hazard identified
Additional information - General Population
etrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (CAS 66669-53-2)
DNEL systemic (worker/general population)
Basis for hazard conclusion systemic effects (worker/General population):
A repeated dose toxicity study in Wistar rats (90 day feeding study) according OECD Guideline 408 is available.
There is no long-term study available using the inhalation or dermal route. Thus, as starting point for the calculation/assessment of hazard, the NOAEL of the sub-chronic feeding study has to be taken into account.
Study
Title: PBS-AM Subchronische toxikologische Untersuchungen an Ratten (Fütterungsversuch über 3 Monate)
Administration period: 90 days
Doses: 50, 200, 1000, 5000 ppm = male rats: 0, 4.17, 14.91, 84.11, 424.41 mg/kg bw/day, female rats: 0, 6.05, 12.51, 125.48, 632.65 mg/kg bw/day
NOAEL, Effects
NOAEL (systemic) = 424 mg/kg bw (male and female rats)
Effects: All doses were tolerated without any effects
A 3-months feeding study was performed in 15 female and 15 male rats with technical tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (50, 200, 1000, 5000 ppm = male rats: 0, 4.17, 14.91, 84.11, 424.41 mg/kg bw/day, female rats: 0, 6.05, 12.51, 125.48, 632.65 mg/kg bw/day). The control animals (30 females, 30 males) received no test substance (0 ppm).
All doses were tolerated without any effects as could be shown by overall observations and examinations: Appearance, behaviour, development, and mortality as well as blood, blood glucose and cholesterol, metabolism of electrolytes (NA, K, Ca), Ferrum and Phosphorus were not affected by doses up to 5000 ppm. The same was proven for the kidneys by urinalyses, clinical chemistry and pathological and histopathological examinations. Gross necropsy and histological examinations did not reveal any adverse effects due to the test substance.
Thus, the NOAEL is equal or higher than 5000 ppm (equivalent to about 424 mg/kg bw for male rats and 632 mg/kg bw for female rats).
Additional, a developmental/teratogenicity study with 2-phosphonobutane-1,2,4-tricarboxylic acid (CAS 37971-36-1) in Wistar rats equivalent or similar to OECD Guideline 414 (Prenatal Developmental Toxicity Study) is available which is used for read-across.
Study Title: Untersuchung auf embryotoxische Wirkungen an Ratten nach oraler Verabreichung [Examination of embryotoxicity effects in rats following oral administration of Bayhibit-AM]
Administration period: 10 days
Doses: 0, 100, 300 or 1000 mg/kg bw/day
NOEL, Effects
NOEL (systemic) = 1000 mg/kg bw
Effects: No maternal toxicity, no teratogenicity, no embryotoxicity was observed under the study conditions.
After oral application of 2-phosphonobutane-1,2,4 -tricarboxylic acid, (CAS 37971-36-1, a structural analogue, please refer to the read across justification document at IUCLID Chapter 13.2) up to a maximal dosage of 1000 mg/kg, no signs of maternal toxicity were found (by means of death, weight loss, changes in appearance and behaviour). Moreover, female mother rats were proved later to be fertile. No influence was observed in embryo and foetus development (resorption, placenta weight, any skeletal and internal malformation). The NOEL value for these effects is therefore determined as 1000 mg/kg bw/day.
Under the experimental conditions, the test item is considered to have no maternal and embryonic toxic effects and no teratogenic effects in rats.
Thus, the NOAEL is equal or higher than 1000 mg/kg bw.
Conclusion: In the sub-chronic study and the developmental/teratogenicity study no adverse effects were observed. Therefore ‘no hazard’ is identified for systemic effects.
Hazard conclusion systemic (worker/general population) short-term
Tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate (CAS 66669-53-2) is not classified for acute oral, acute dermal and acute inhalation toxicity.
Conclusion: Therefore ‘no hazard’ is identified for systemic effects (short term).
Hazard conclusion local effects (worker/general population)
Basis for hazard conclusion local (long- and short-term toxicity):
Irritation/corrosion
In the key studies for skin irritation/corrosion and eye irritation, the test substance (tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate) is not irritating to the skin and eyes.
A classification is not justified.
Sensitization
In a Guinea Pig maximization test (GPMT) the test substance (tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate) revealed no sensitisation potential.
A classification is therefore not justified.
Conclusion: For local effects no hazard is identified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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