O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to US EPA guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 123-1 (Seedling Emergence Tier II)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

EC Number:

255-255-2

EC Name:

O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

Cas Number:

41198-08-7

Molecular formula:

C11H15BrClO3PS

IUPAC Name:

4-bromo-2-chlorophenyl ethyl (propylsulfanyl)phosphonate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test organisms

1

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Soybean- Plant family: Leguminosae- Variety: Forrest- Source of seed: Azlin Seed Company

2

Species:

Lactuca sativa

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Lettuce- Plant family: Compositae- Variety: Prize Head, Zero M-1- Source of seed: Germain's

3

Species:

Daucus carota

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Carrot- Plant family: Umbelliferae- Variety: Danvers Half Long- Source of seed: Germain's

4

Species:

Lycopersicon esculentum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Tomato- Plant family: Solanaceae- Variety: VF 6203- Source of seed: Petoseed

5

Species:

Cucumis sativus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Cucumber- Plant family: Curcubitaceae- Variety: Bush Champion- Source of seed: Burpee Seed Company

6

Species:

Brassica oleracea var. capitata

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Cabbage- Plant family: Cruciferae- Variety: Earliana- Source of seed: Burpee Seed Company

7

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Oat- Plant family: Gramineae- Variety: Swan- Source of seed: Germain's

8

Species:

Lolium perenne

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Perennial ryegrass- Plant family: Gramineae- Variety: Linn- Source of seed: Germain's

9

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Corn- Plant family:Gramineae- Variety: Golden Cross Bantam- Source of seed: Burpee Seed Company

10

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Onion- Plant family: Amaryllidaceae- Variety: Stockton Early Yellow- Source of seed: Germain's

Study design

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

natural soil

Total exposure duration:

21 d

Test conditions

Test temperature:

20-30°C

Moisture:

Pots were irrigated daily recieving approximately 53 mL water each day

Details on test conditions:

TEST SYSTEM- Testing facility: Laboratory Greenhouse- Test container: Plastic pot 7.5 x 7.5 x 6.0 cm- Amount of soil: Each pot was completely filled with soil- Method of seeding: Soybean, cucumber, oat and corn were planted at a depth of 2.5 cm while the remaining 6 species were planted at a depth of 1.3 cm. A plexiglass template was used to create planting holes in the soil- No. of seeds per container: 10- No. of replicates per treatment group: 3- No. of replicates per control: 3- No. of replicates per vehicle control:SOURCE AND PROPERTIES OF SUBSTRATE (if soil)Hanford Sandy Loam soil withadded perlite. Soil had been sterilised prior to the test. GROWTH CONDITIONS- Volume water applied:53 mL/ day approximately- 23.3 mL applied as part of treatment application. - Method of application: Material applied with a belt sprayer from within a fume hood. Phytotoxicity: Plants were observed for signs of phytotoxicity, and the plant height was measured.

Nominal and measured concentrations:

Nominal test concentrations: 0, 0.123, 0.037, 0.111, 0.333 and 1 .0 lb/AEquivalent to 0, 0.0138, 0.0414, 0.124, 0.373 and 1.12 kg/ha

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 2

Species:

Lactuca sativa

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 3

Species:

Daucus carota

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 4

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 5

Species:

Brassica oleracea var. capitata

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 6

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 7

Species:

Lolium perenne

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 8

Species:

Zea mays

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 9

Species:

Allium cepa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.12 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Effect concentrations 10

Species:

Cucumis sativus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

1 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Details on results:

SEEDLING EMERGENCEAt the 21 day observation period there were no significant differances in seedling emergence in any of the treatment rates for soybean, lettuce, carrot, tomato, cabbage, oat, ryegrass, corn, and onion. Cucumber was affected at th e ctreatment rate of 1.12 kg/ha. The EC50 value for cucumber was determined to be 1 kg/ha. Cucumber was also the only species to show significant phytotoxic effects at the 1.12 kg/ha treatment group though not at the lower application rates.. .