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EC number: 266-442-3 | CAS number: 66669-53-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in soil
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in soil: simulation testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1995-10-05 to 1996-05-30
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The deviation to OECD test guideline 304A is restricted to usage of different concentrations and duration of the test.
- Justification for type of information:
- Please find the Justification attached to Chapter 13.2.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 304 A (Inherent Biodegradability in Soil)
- Deviations:
- yes
- Remarks:
- e.g. amount of radioactivity applied/assessed, duration of the study
- Principles of method if other than guideline:
- In general the principle of the method applied is identical to the method stated in OECD guideline 304A. Only details of implementation are not in all aspects identical.
- GLP compliance:
- not specified
- Test type:
- laboratory
- Radiolabelling:
- yes
- Oxygen conditions:
- aerobic
- Soil classification:
- other: German standard soils BBA 2.1 (sand), BBA 2.2 (loamy sand) and silt loam from Bayer farm Laacherhof
- Year:
- 1 995
- Soil no.:
- #1
- Soil type:
- sand
- % Org. C:
- 2.48
- CEC:
- 5 meq/100 g soil d.w.
- Soil no.:
- #2
- Soil type:
- loamy sand
- % Org. C:
- 10
- CEC:
- 10 meq/100 g soil d.w.
- Soil no.:
- #3
- Soil type:
- silt loam
- % Org. C:
- 0.9
- CEC:
- 8 meq/100 g soil d.w.
- Details on soil characteristics:
- German standard soils 1.) BBA 2.1 (sand), 2.) BBA 2.2 (loamy sand) and 3.) silt loam from Bayer farm Laacherhof
- Soil No.:
- #1
- Duration:
- ca. 133 d
- Soil No.:
- #2
- Duration:
- ca. 133 d
- Soil No.:
- #3
- Duration:
- ca. 133 d
- Soil No.:
- #1
- Initial conc.:
- 0.92 ppm
- Based on:
- test mat.
- Soil No.:
- #2
- Initial conc.:
- 0.92 ppm
- Based on:
- test mat.
- Soil No.:
- #3
- Initial conc.:
- 0.92 ppm
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Soil No.:
- #1
- Temp.:
- 20
- Humidity:
- 14.94 g water in 100 g dry soil
- Microbial biomass:
- 74 mg microbial C/kg DW soil (initial), 21 mg microbial C/kg DW soil (end)
- Soil No.:
- #2
- Temp.:
- 20
- Humidity:
- 22.38 g water in 100 g dry soil
- Microbial biomass:
- 406 mg microbial C/kg DW soil (initial), 181 mg microbial C/kg DW soil (end)
- Soil No.:
- #3
- Temp.:
- 20
- Humidity:
- 17.50 g water in 100 g dry soil
- Microbial biomass:
- 531 mg microbial C/kg DW soil (initial), 249 mg microbial C/kg DW soil (end)
- Details on experimental conditions:
- About one week before degradation test start the soils were gently air-dried so that they could be screened to <= 2 mm. Subsequently, the soil moisture (% of dry weight, DW) was determined. Each 100 g DW were weighed into 300 ml Erlenmeyer flasks and then adjusted to a soil moisture of 50% of the maximum water holding capacity (WHC max.) each by using demineralised water. The volume of 1 ml water later added via the application solution was taken into account (for data see Table 2). The test batches were weighed, closed with cot-ton wool and pre-incubated at 20°C in the dark until the beginning of the test (4 days later). For respective data see following Table 2. Determinations of the microbial population of soils were performed by the laboratory of Dr. J.P.E. Anderson, Bayer AG, PF-E/OE, bldg. 6620, Monheim at the start and at the end of the experiment
- Soil No.:
- #1
- % Recovery:
- 104.6
- Remarks on result:
- other: soil BBA 2.1 (sand)
- Soil No.:
- #2
- % Recovery:
- 101.4
- Remarks on result:
- other: soil BBA 2.2 (loamy sand)
- Soil No.:
- #3
- % Recovery:
- 102.8
- Remarks on result:
- other: silt loam, Bayer farm Laacherhof
- Parent/product:
- parent
- Soil No.:
- #1
- % Degr.:
- ca. 21
- Parameter:
- CO2 evolution
- Sampling time:
- 133 d
- Parent/product:
- parent
- Soil No.:
- #2
- % Degr.:
- ca. 27
- Parameter:
- CO2 evolution
- Sampling time:
- 133 d
- Parent/product:
- parent
- Soil No.:
- #3
- % Degr.:
- ca. 15
- Parameter:
- CO2 evolution
- Sampling time:
- 133 d
- Key result
- Soil No.:
- #1
- DT50:
- ca. 142 d
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Soil No.:
- #2
- DT50:
- ca. 102 d
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Soil No.:
- #3
- DT50:
- ca. 107 d
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Transformation products:
- not measured
- Details on transformation products:
- NA
- Evaporation of parent compound:
- not measured
- Volatile metabolites:
- not measured
- Residues:
- not measured
- Details on results:
- The degradability of [3,4-14C]PBTC was investigated in three agricultural soils: 1.) BBA 2.1 (sand), 2.) BBA 2.2 (loamy sand), 3.) silt loam from Bayer farm Laacherhof. Start concentration was 0.92 µg PBTC/100 g DW of soil (0.92 ppm). Temperature and soil moisture during testing period (133 days) were 20°C and ca. 50% of the resp. maximum water holding capacity. The recoveries (material balances) ranged from 101.7% to 105.6%. The [14C]PBTC was metabolised to 14CO2, the main degradation product, accounting for 21.3% , 27.4%, 15.5% of the applied radioactivity in the soils 1.), 2.) and 3.) after 133 days, respectively. During the incubation period a constant increase of 14CO2 was measured. However, decreased 14CO2-formation rates with increasing time parallel to the decrease of the active biomass of soils were noted.
After 133 days low portions of PBTC were recovered by two extractions using aqueous CaCl2 solution (2.1%, 1.4% and 0.2% for soils BBA 2.1, BBA 2.2 and Laacherhof, respectively) indicating a correlation to the textural class of soil. The main portion of radioactivity (pre-dominantly as PBTC) was extracted by extensive HCl extraction. The portion of not-extracted (bound) residue resulting from the [14C]PBTC treatment amounted to 16.8%, 31.8% and 42.1% for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively. Correlation to the textural class of soil (lowest bound residues in the sand, highest in the silt loam) was observed. The predominant portion of radioactivity as well as of PBTC remaining in soil after 133 days of incubation was not easy to extract indicating a low mobility or leaching potential of PBTC in soils.
The time for disappearance of 50% of PBTC (DT50 value) calculated (1st order) from the results of HPLC (on realistic worst case assumption for peak evaluation) was 142 days, 102 days and 107 days for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively.
Due to known limitations of laboratory test systems (not all the processes relevant for degradation under outdoor conditions are reflected) the degradation rates reported here do not necessarily reflect the real situation in a natural environment. Nevertheless, it was shown that the PBTC is moderately degradable and is thoroughly metabolised to CO2 in soil. - Results with reference substance:
- NA
- Conclusions:
- Degradability of radioactive labelled [14C]PBTC was investigated in three soils following the OECD guideline 304A. Main degradation product was 14CO2, on average between 15.5 and 27.4 % after 133 d. DT50 between 102 and 142 days were calculated. The recoveries (material balances) for the different test vessels ranged from 101.7 to 105.6 %. It was shown that PBTC is moderately degradable and thoroughly metabolised to CO2 in soil. However, with a worst-case DT50 >142 d the substance must be classified as persistant (P).
- Executive summary:
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate ("PBTCNa4")a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid ("PBTC") is proposed.
In aqueous media, PBTCNa4 and PBTC dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of PBTC and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.
Therefore a read-across between PBTCNa4 and PBTC is justified.
The degradability of [3,4-14C]PBTC was investigated in three agricultural soils following the OECD guideline 304A. The test soils maintained under aerobic conditions were German standard soils 1.) BBA 2.1 (sand), 2.) BBA 2.2 (loamy sand), 3.) silt loam from Bayer farm Laacherhof. Start concentration was 0.92 µg PBTC/100 g DW of soil (0.92 ppm). Temperature and soil moisture during total testing period of 133 days were 20°C and about 50% of the respective maximum water holding capacity. The recoveries (material balances) for the different test vessels ranged from 101.7% to 105.6%.
The [14C]PBTC was thoroughly metabolised to 14CO2, the main degradation product, accounting for 21.3% , 27.4%, 15.5% of the applied radioactivity in the soils 1.), 2.) and 3.) after 133 days, respectively. During the incubation period a constant increase of 14CO2 was measured. However, the formation rates of 14CO2 decreased with increasing time parallel to the decrease of the active biomass of soils.
After 133 days low portions of PBTC were recovered by two extractions using aqueous CaCl2 solution (2.1%, 1.4% and 0.2% for soils BBA 2.1, BBA 2.2 and Laacherhof, respectively) indicating a correlation to the textural class of soil. The main portion of radioactivity (pre-dominantly as PBTC) was extracted by extensive HCl extraction. The portion of not-extracted (bound) residue resulting from the [14C]PBTC treatment amounted to 16.8%, 31.8% and 42.1% for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively. Correlation to the textural class of soil (lowest bound residues in the sand, highest in the silt loam) was observed. The predominant portion of radioactivity as well as of PBTC remaining in soil after 133 days of incubation was not easy to extract indicating a low mobility or leaching potential of PBTC in soils.
The time for disappearance of 50% of PBTC (DT50 value) calculated (1st order) from the results of HPLC (on realistic worst case assumption for peak evaluation) was 142 days, 102 days and 107 days for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively.
Due to known limitations of laboratory test systems (not all the processes relevant for degradation under outdoor conditions are reflected) the degradation rates reported here do not necessarily reflect the real situation in a natural environment. It was shown that the PBTC is moderately degradable and is thoroughly metabolised to CO2 in soil. However, with a worst-case DT50 of 142 d the substance must be classified as persistant (P).
Reference
No remarks.
Description of key information
Degradability of the radioactive labelled parent acid [14C]PBTC was investigated in three soils. Main degradation product was 14CO2, on average between 15.5 and 27.4 % after 133 d. DT50 between 102 and 142 days were calculated. The recoveries (material balances) for the different test vessels ranged from 101.7 to 105.6 %. It was shown that PBTC is moderately degradable and thoroughly metabolised to CO2 in soil. However, with a worst-case DT50 of 142 d the substance must be classified as persistant (P).
Key value for chemical safety assessment
- Half-life in soil:
- 142 d
- at the temperature of:
- 20 °C
Additional information
As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate ("PBTCNa4") a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid ("PBTC") is proposed. In aqueous media, PBTCNa4 and PBTC dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of PBTC and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations. Therefore a read-across between PBTCNa4 and PBTC is justified.
The degradability of [3,4-14C]PBTC was investigated in three agricultural soils following the OECD TG 304A. The test soils maintained under aerobic conditions were German standard soils 1.) BBA 2.1 (sand), 2.) BBA 2.2 (loamy sand), 3.) silt loam from Bayer farm Laacherhof. Start concentration was 0.92 µg PBTC/100 g DW of soil (0.92 ppm). Temperature and soil moisture during total testing period of 133 days were 20°C and about 50% of the respective maximum water holding capacity. The recoveries (material balances) for the different test vessels ranged from 101.7% to 105.6%.
The [14C]PBTC was thoroughly metabolised to 14CO2, the main degradation product, accounting for 21.3% , 27.4%, 15.5% of the applied radioactivity in the soils 1.), 2.) and 3.) after 133 days, respectively. During the incubation period a constant increase of 14CO2 was measured. However, the formation rates of 14CO2 decreased with increasing time parallel to the decrease of the active biomass of soils.
After 133 days low portions of PBTC were recovered by two extractions using aqueous CaCl2 solution (2.1%, 1.4% and 0.2% for soils BBA 2.1, BBA 2.2 and Laacherhof, respectively) indicating a correlation to the textural class of soil. The main portion of radioactivity (pre-dominantly as PBTC) was extracted by extensive HCl extraction. The portion of not-extracted (bound) residue resulting from the [14C]PBTC treatment amounted to 16.8%, 31.8% and 42.1% for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively. Correlation to the textural class of soil (lowest bound residues in the sand, highest in the silt loam) was observed. The predominant portion of radioactivity as well as of PBTC remaining in soil after 133 days of incubation was not easy to extract indicating a low mobility or leaching potential of PBTC in soils.
The time for disappearance of 50% of PBTC (DT50 value) calculated (1st order) from the results of HPLC (on realistic worst case assumption for peak evaluation) was 142 days, 102 days and 107 days for the soils BBA 2.1, BBA 2.2 and Laacherhof, respectively.
Due to known limitations of laboratory test systems (not all the processes relevant for degradation under outdoor conditions are reflected) the degradation rates reported here do not necessarily reflect the real situation in a natural environment. It was shown that the PBTC is moderately degradable and is thoroughly metabolised to CO2 in soil. However, with a worst-case DT50 of 142 d the substance must be classified as persistant (P).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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