Cetalkonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute toxicity test was carried out for the test chemical on Daphnia magna

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: water flea
- Age at study initiation: 24 h old

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Hardness:

250 mg/l (as CaCO3)

Test temperature:

20°C

Nominal and measured concentrations:

10 concentrations in a geometric series were tested

Details on test conditions:

- Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration.

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

IC50

Effect conc.:

0.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% Confidence range 0.18- 0.26 mg/l

Conclusions:

The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l

Executive summary:

Acute toxicity test were carried out on Daphnia magna according to OECD guideline Daphnia magna, laboratory bred, not more than 24 h old were used in this test, where the swimming incapabilility is the end point. Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration 10 concentrations in a geometric series were tested in the concentration range first established in a preliminary test. The percentage immobility at 24 h was plotted against concentration on logarithmic-probability paper, a linear relationship was obtained and the IC50 was calculated from the corresponding equation.

 

The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l. The test material is considered to be toxic to aquatic environment .Since , the test material is readily biodegradable in aquatic environment , it can be classified as aquatic acute 1 as per CLP criteria.  

Description of key information

Short term toxicity to aquatic invertebrates:

Acute toxicity test were carried out on Daphnia magna according to OECD guideline Daphnia magna, laboratory bred, not more than 24 h old were used in this test, where the swimming incapabilility is the end point. Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration 10 concentrations in a geometric series were tested in the concentration range first established in a preliminary test. The percentage immobility at 24 h was plotted against concentration on logarithmic-probability paper, a linear relationship was obtained and the IC50 was calculated from the corresponding equation.

 The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l. The test material is considered to be toxic to aquatic environment .Since , the test material is readily biodegradable in aquatic environment , it can be classified as aquatic acute 1 as per CLP criteria.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.22 mg/L

Additional information

Short term toxicity to aquatic invertebrates:

Toxicity of test material was evaluated for its potentail to cause immobility on test aqutic invertebrate based on the data provided in peer reviewed journal and authoritative database as mentioned below:

1) Acute toxicity test were carried out on Daphnia magna according to OECD guideline Daphnia magna, laboratory bred, not more than 24 h old were used in this test, where the swimming incapabilility is the end point. Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration 10 concentrations in a geometric series were tested in the concentration range first established in a preliminary test. The percentage immobility at 24 h was plotted against concentration on logarithmic-probability paper, a linear relationship was obtained and the IC50 was calculated from the corresponding equation.

 The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l. The test material is considered to be toxic to aquatic environment .Since , the test material is readily biodegradable in aquatic environment , it can be classified as aquatic acute 1 as per CLP criteria.  

2)Short term toxicity study for test material was performed . The effect concentration LC50 after 24 h for daphnia magna was observed to be 0.18 mg/l. Based on the above effect concentartion it can be considered that test chemical is highly toxic to aquatic invertebrate and can be clasiffied as aquatic chornic 1

3)The test chemical was used to evaluate short-term toxicity on aquatic invertebrate .>24 h old Daphnia magna were used for the test .The test was carried out under static condition for 24 hrs.Behavior changes were observed after 24 h The effect concentration (EC50) of test chemical after 24 h on Daphnia magna was observed to be 0.180 mg/l.Hence, based on the above effect concentration test chemical can be classified as "Aquatic acute 1".

Based on the above effect concentration value it can be clasiffied as "Aquatic acute 1".