Cetalkonium chloride

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Acute toxicity test were carried out on Daphnia magna according to OECD guideline Daphnia magna, laboratory bred, not more than 24 h old were used in this test, where the swimming incapabilility is the end point. Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration 10 concentrations in a geometric series were tested in the concentration range first established in a preliminary test. The percentage immobility at 24 h was plotted against concentration on logarithmic-probability paper, a linear relationship was obtained and the IC50 was calculated from the corresponding equation.

 The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l. The test material is considered to be toxic to aquatic environment .Since , the test material is readily biodegradable in aquatic environment , it can be classified as aquatic acute 1 as per CLP criteria.  

Toxicity to aquatic algae and cyanobacteria:

A short term toxicity test for test chemical test chemical was performed on C. vulgaris for 96 h .The effect concentration (EC50) of test material on C. vulgaris was observed to be 0.161 mg/l. Based on the above effect concentration it can be considered that the test substance is highly toxic to aquatic algae and hence, can be clasiffied as aquatic acute 1.

Toxicity to microorganism:

Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.

Additional information

Short term toxicity to aquatic invertebrates:

Toxicity of test material was evaluated for its potentail to cause immobility on test aqutic invertebrate based on the data provided in peer reviewed journal and authoritative database as mentioned below:

1) Acute toxicity test were carried out on Daphnia magna according to OECD guideline Daphnia magna, laboratory bred, not more than 24 h old were used in this test, where the swimming incapabilility is the end point. Twenty Daphnia, divided into four groups of five animals each, was used at each test concentration 10 concentrations in a geometric series were tested in the concentration range first established in a preliminary test. The percentage immobility at 24 h was plotted against concentration on logarithmic-probability paper, a linear relationship was obtained and the IC50 was calculated from the corresponding equation.

 The effect concentration of test material on daphnia magna after 24 h was observed to be 0.22 mg/l and the 95% Confidence range 0.18 – 0.26 mg/l. The test material is considered to be toxic to aquatic environment .Since , the test material is readily biodegradable in aquatic environment , it can be classified as aquatic acute 1 as per CLP criteria.  

2)Short term toxicity study for test material was performed . The effect concentration LC50 after 24 h for daphnia magna was observed to be 0.18 mg/l. Based on the above effect concentartion it can be considered that test chemical is highly toxic to aquatic invertebrate and can be clasiffied as aquatic chornic 1

3)The test chemical was used to evaluate short-term toxicity on aquatic invertebrate .>24 h old Daphnia magna were used for the test .The test was carried out under static condition for 24 hrs.Behavior changes were observed after 24 h The effect concentration (EC50) of test chemical after 24 h on Daphnia magna was observed to be 0.180 mg/l.Hence, based on the above effect concentration test chemical can be classified as "Aquatic acute 1".

Based on the above effect concentration value it can be clasiffied as "Aquatic acute 1".

Toxicity to aquatic algae and cyanobacteria:

Toxicity of test material was evaluated for its potentail to inhibit growth of aquatic algae based on the data provided in peer reviewed journal and authoritative database as mentioned below:

1)A short term toxicity test for test chemical test chemical was performed on C. vulgaris for 96 h .The effect concentration (EC50) of test material on C. vulgaris was observed to be 0.161 mg/l. Based on the above effect concentration it can be considered that the test substance is highly toxic to aquatic algae and hence, can be clasiffied as aquatic acute 1.

2)A short term toxicity test for test chemical test chemical was performed on C. vulgaris for 96 h .The effect concentration (EC50) of test material on C. vulgaris was observed to be 0.15 mg/l. Based on the above effect concentration it can be considered that the test substance is highly toxic to aquatic algae and hence, can be clasiffied as aquatic acute 1.

3)Test material was used to evaluate toxicity to aquatic algae Chlorella pyrenoidosa . The test was carried out under static condition for 96h at the temperature 24 deg C . The effect concentration of test material on green algae Chlorella pyrenoidosa was observed to be 0.17 mg/l.Hence, based on the above effect concentration test chemical can be classified as "Aquatic acute 1".

Toxicity to microorganism:

Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.