Cetalkonium chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge nitrification inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

luminiscence reduction test was performed for test material on Photobacterium phosphoreum

GLP compliance:

not specified

Test organisms (species):

other: P. phosphoreum

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

15 deg C

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

0.22 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: luminiscent

Conclusions:

The effect concentration EC50 of test material on P. phosphoreum was evaluated to be 0.22 mg/l

Executive summary:

Microtox bioassay was performed for the test material on P. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reduction of the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactant solution at 15 deg C. The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l

Description of key information

Toxicity to microorganism:

Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.

Additional information

Toxicity to microorganism:

Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.