Propylidynetrimethanol, propoxylated, reaction products with ammonia

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000-04-27 to 2000-06-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

liquid

Details on test material:

Name of test material: Trimethylolpropanepoly(oxypropylene)triamine
EC no.: 500-105-6
CAS no.: 39423-51-3
Physical state: clear colourless liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 8T509 (Huntsman Polyurethanes, Belgium)
- Analytical purity: 90.1% primary amine, as a percentage of total amine

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In the dark, at ambient room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Buckland Sewage Treatment Works, Newton Abbot, Devon, UK on 25 April 2000; predominantly domestic sewage treated at this facility
- Storage conditions: Kept aerated at room temperature and pH maintained at 7.0 +/- 1.0
- Storage length: 9 days
- Preparation of inoculum for exposure: Centrifuged, washed, and resuspended in test medium and solids concentration determined 7 days prior to test.
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

BOD

Details on study design:

TEST CONDITIONS
- Composition of medium: Standard media
- Test temperature: 22 +/- 2 °C
- pH: 7.0 +/- 1.0
- pH adjusted: yes
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 3
- Measuring equipment: Hach manometric biochemical oxygen demand apparatus- Dark 500 ml bottle on a magnetic stirrer connected to a closed end mercury manometer.
- Details of trap for CO2 and volatile organics if used: KOH in a seal cup in the neck of each bottle.

SAMPLING
- Sampling frequency: Oxygen uptake was recorded 5 times/week. Readings corrected for the blank and the volume of test solution.

Reference substance

Reference substance:

other: sodium acetate

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

5 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

11 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

15 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

20 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The measured COD value of sodium acetate was 0.66 g O2 per g test substance. This is comparable to its expected value, and validates the technique. The maximum biodegradation level attained was 71%, 60% of which occurred within the 10 day-window as expected for a biodegradable substance, thus confirming that the activated sludge contained viable organisms.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance was not readily biodegradable under the conditions of the test.