Hydrochloric acid, reaction products with aniline and nitrobenzene, sulfonated, sodium salts

Endpoint summary

Additional information

Short-term toxicity to fish

In a static limit test system a 96h-LC0 of ≥ 100 mg/L was determined on Danio rerio related to nominal concentration. Analytical monitoring was carried out and the nominal concentrations were confirmed. As a consequence it can be concluded the 96h-LC50 is > 100 mg/L. The test was conducted according to the Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).

Short-term toxicity to Daphnia

Daphnia magna was investigated in a static limit test in accordance with the Council Regulation (EC) No 440/2008, Method C.2 'Acute toxicity for Daphnia' which is equivalent to OECD Guideline for Testing of Chemicals No. 202. Acute Immobilisation Test' (adopted April 13, 2004). No toxic effect was observed and a 48h-EC50 of >100 mg/L was received. The LC50-value relates to nominal concentration as effective concentrations correspond to 95.1 % of nominal values at 0 hours and to 93.4 % of nominal values at 48 hours.

Toxicity to aquatic algae

The acute toxicity of the substance to alga (Desmodesmus subspicatus) was tested according to the EU Method C.2 (Acute Toxicity for Daphnia), which is equivalent to OECD Guideline for Testing of Chemicals No. 201. After 72 hours an EC50 of 89.8 mg/L, a NOEC of 3.4 mg/L and a LOEC of 20.4 mg/L. The results are expressed in terms of geometric mean measured concentrations.

The substance is dark coloured and reduce the light emission for the algae cells because of shading effects. So it is conceivable that the result of this alga growth inhibition test is not due to the chemical toxic effect of the substance, but is rather caused by shading effects. Therefore the results should be handled with care. In an alternative approach to evaluate the EC50 of growth rate without the NOEC/LOEC calculation, only the highest concentration have to be considered. According to the analytical results an evaluation based on the nominal concentration for the concentration of 100 mg/L substance is satisfactorily because the exposure concentrations vary less than 20% from the nominal values during the test. With this alternative approach these values result in an EC50 value higher than 100 mg/L: 72h-EC50 >100 mg/L.

Toxicity to microorganisms

To assess the toxicity of the substance to microorganisms a study was conducted according to the EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test) which is equivalent to OECD Guideline for Testing of Chemicals No.201. The substance showed no respiration inhibition of activated sludge at a concentration of 1000 mg/L. The EC50 is higher than 1000 mg/L. The effect value relates to nominal concentration, since no analytical monitoring was performed.