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EC number: 225-969-9 | CAS number: 5188-07-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium methanethiolate
- EC Number:
- 225-969-9
- EC Name:
- Sodium methanethiolate
- Cas Number:
- 5188-07-8
- Molecular formula:
- CH4S.Na
- IUPAC Name:
- sodium methylsulfanide
- Details on test material:
- Test article name : Sodium methylmercaptide
CAS RN°: 5188-07-8
Origin: Elf Atochem, Rotterdam
Batch: 462
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 102, TA 98, TA 100
- Additional strain / cell type characteristics:
- other: test organisms were properly maintained and were checked for appropriate genetic markers (rfa mutation, R factor)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction from liver homogenates from rats induced with Aroclor 1254 (500 mg/kg) by intraperitoneal route
- Test concentrations with justification for top dose:
- The concentration mentioned by the laboratory performing the study is related to the test article as such (sodium methyl mercaptan 31.4 % w/w). However, the toxicity, variable in strains, make impossible to use higher concentrations.
(a) Preliminary cytotoxicity assay: Plate incorporation assay: 0, 10, 100, 1000, 2500 and 5000 µg/plate were evaluated with and without S9 activation in TA100. A single plate was used, per dose, per condition.
(b)Mutation assays: .
Plate incorporation assay:
0, 312.5, 625, 1250, 2500 or 5000 µg/plate for all test strains with and without metabolic activation.
Pre-incubation assay:
0, 312.5, 625, 1250, 2500 or 5000 µg/plate for TA1535, TA1537 and TA100 or 0, 125, 250, 500, 1000 or 2000 µg/plate for TA 102 and TA 98, without metabolic activation.
0, 312.5, 625, 1250, 2500 or 5000 µg/plate for TA1535, TA1537, TA100 and TA102 or 0, 312.5, 625, 1250, 2500 or 4000 µg/plate for TA 102, with metabolic activation. - Vehicle / solvent:
- Distilled water
Controls
- Untreated negative controls:
- yes
- Remarks:
- culture medium
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water (50 mg/mL)
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- All positive controls were diluted in dimethyl sulfoxide.
- Positive control substance:
- other: With S9: 2-anthramine 2.0 µg/plate (TA98-TA100-TA1535-TA1537 ), Danthron 30 µg/plate ( TA102 ). Without S9: 2-nitrofluorene 0.5 µg/plate(TA98 ), Sodium azide 1 µg/plate (TA100-TA1535), 9-aminoacridine 50 µg/plate (TA1537), Mitomycin 0.5 µg/plate (TA102)
- Details on test system and experimental conditions:
- PRELIMINARY TOXICITY ASSAY
The preliminary toxicity assay was used to establish the dose range over which the test article would be assayed.
MUTAGENICITY ASSAY
Five dose levels of test article along with appropriate vehicle control and positive controls were plated with overnight cultures of TA98, TA100, TA1535, TA1537 and TA102 on selective agar in the presence and absence of Aroclor induced rat liver S9. All dose levels of test article, vehicle control and positive controls were plated in triplicate.
TEST PROCEDURE
Plate incorporation method: One half (0.5) milliliter of S9 or Sham mix, 100 µL of tester strain and 100 µL of vehicle or test article dilution were added to 2.0 mL of molten selective top agar at 45°C. After vortexing, the mixture was overlaid onto the surface of minimal bottom agar.
Preincubation method: One-half (0.5) milliliter of S9 or sham mix, 100 µL of tester strain and 100 µL of vehicle or test article dilution were added to glass culture tubes. Alter vortexing, these mixtures were incubated with shaking for 60 minutes at 37°C. Following the preincubation, 2.0 mL of selective top agar was added to each tube and the mixture was vortexed and overlaid onto the surface of minimal bottom agar. When plating the positive controls, the test article aliquot was replaced by the of appropriate positive control. After the overlay had solidified, the plates were inverted and incubated for approximately 48 to 72 hours at 37°C.
Revertant colonies for a given tester strain and activation condition were counted by automated colony counter. - Evaluation criteria:
- Data sets for a tester strain was judged positive if the increase in mean revertants at one or more of the tested concentrations is equal to or greater than 2.0-times the mean vehicle control value.
- Statistics:
- none
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 102, TA 98, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Without S9: >= 1000 µg/plate. With S9: >= 2500 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The negative and solvent control results were equivalent to
those usually obtained in the Laboratory. The number of
revertants induced by the positive controls was higher than
the spontaneous one, which demonstrated the sensitivity of
this test and the efficacy of the S9 mix throughout this
study.
The test substance SODIUM METHYL MERCAPTIDE did not induce
any significant increase in the revertant number with or
without S9 mix in any of the 5 strains.
Mean Number of Revertants Per Plate
Activation: None
Dose (µg/plate) |
TA98 |
TA100 |
TA102 |
TA1535 |
TA1537 |
Ctrl |
2 |
7 |
370* |
14 |
15 |
312.5 |
5 |
5 |
478 |
16 |
16 |
625 |
3 |
7 |
400 |
13 |
17 |
1250 |
18(+++) |
5 |
388 |
16 |
16 |
2500 |
5(++) |
5 |
347(+++) |
19 |
13 |
5000 |
18 (+++) |
5 |
229(+++) |
20(+) |
15 |
Positive Control |
103 |
43 |
1996 |
490 |
348 |
*Spontaneous revertants rather than solvent control, study
error
Dose (µg/plate) |
TA100 |
TA1535 |
TA1537 |
Ctrl |
102 |
16 |
12 |
312.5 |
93 |
24 |
18 |
625 |
97 |
21 |
14 |
1250 |
94(+) |
19 |
9 |
2500 |
90(++) |
20 |
17 |
5000 |
64(+++) |
18(+) |
8 |
Positive Control |
658 |
461 |
180 |
Dose (µg/plate) |
TA98 |
TA102 |
Ctrl |
32 |
284 |
125 |
43 |
294 |
250 |
34 |
320 |
500 |
27 |
362 |
1000 |
34(++) |
325(++) |
2000 |
13(++) |
242(+++) |
Positive Control |
1173 |
1335 |
Activation: S9, Direct plate incorporation
Dose (µg/plate) |
TA98 |
TA100 |
TA102 |
TA1535 |
TA1537 |
Ctrl |
33 |
122 |
505 |
14 |
14 |
312.5 |
28 |
139 |
607 |
20 |
20 |
625 |
35 |
128 |
551 |
21 |
19 |
1250 |
41 |
139 |
457 |
16 |
20 |
2500 |
36 |
131 |
578 |
16 |
16 |
5000 |
35 |
120 |
446 |
16 |
15 |
Positive Control |
1211 |
1765 |
984 |
263 |
115 |
Activation: S9, Plate - preincubation
Dose (µg/plate) |
TA98 |
TA100 |
TA102 |
TA1535 |
TA1537 |
Ctrl |
42 |
113 |
375 |
23 |
15 |
312.5 |
48 |
94 |
431 |
21 |
24 |
625 |
38 |
97 |
489 |
21 |
20 |
1250 |
47 |
99(+) |
513 |
27 |
24 |
2500 |
47(++) |
94(++) |
530 |
26 |
21 |
5000 |
0(+++) |
0(+++) |
409(+) |
0(+++) |
0(+++) |
Positive Control |
952 |
1217 |
1040 |
338 |
148 |
( ) Parentheses denote degree of toxicity noted at these concentration
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
A Salmonella typhimuriumreverse mutation assay was performed with sodium methanethiolate according to OECD Guideline 471. S. typhimuriumstrains, TA98, TA100, TA102, TA1535 and TA1537, were exposed to five concentrations of sodium methanethiolate in the presence and absence of a metabolic activation system using the plate incorporation and the preincubation methods. All doses of sodium methanethiolate, vehicle control and positive controls were plated in triplicate and incubated for 48-72 hours at 37 ºC. The positive controls gave expected responses. Sodium methanethiolate was cytotoxic at ≥1000 and ≥2500 µg/plate, in the absence and presence of a metabolic activation system, respectively. Treatment of the S. typhimuriumstrains with sodium methanethiolate in the presence or absence of metabolic activation did not induce any significant increases in the revertant numbers.
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