Reaction mass of 2-(methylamino)ethanol, compound with sulphur dioxide and bis[(2-hydroxyethyl)ammonium] sulphite

Endpoint summary

Additional information

Reaction mass of bis(2-hydroxyethanaminium) sulfite and 2-(methylamino)ethanol - oxosulfane oxide (1:1) is a liquid with a small molecular weight and high water solubility at room temperature (MW = 204.24, water solubility = unlimited miscibility). These physicochemical properties indicate a good bioavailability via the dermal and oral route of the substance.

Systemic effects after acute exposure show that Reaction mass of bis(2-hydroxyethanaminium) sulfite and 2-(methylamino)ethanol - oxosulfane oxide (1:1) becomes bioavailable. In the acute oral toxicity study (BASF, 1980), 2/10 rats at 3160 mg/kg, 6/10 rats at 3830 mg/kg, and 10/10 rats at 5000 mg/kg died. Clinical signs were: dyspnoea, apathy, stagger, spastic gait, ruffled fur, poor general condition, abnormal posture, paresis, yellow urine, and cyanosis, indicating systemic availability. In addition, Reaction mass of bis(2-hydroxyethanaminium) sulfite and 2-(methylamino)ethanol - oxosulfane oxide (1:1) was found to be irritating to the skin (BASF, 1980) facilitating dermal uptake.

For further assessment, a weight of evidence approach with methylethanolamin, 2-aminoethanol, and sulfites will be done.

In a teratology study, monomethylethanolamine-supplementation resulted in elevated levels of both choline (43%) and acetylcholine (27%) when compared to levels detected in the choline-deficient derived pups (Zahniser et al., 1978). Measurable (11.7±1.8 nmol/g) amounts of dimethylethanolamine (DMAE) were detected in the brains of monomethylethanolamine-exposed pups (one-day-old). In addition, phosphatidylcholine and phosphatidyl aminoethanol levels in the brain were significantly (p<0.05) lower relative to the choline-deficient-fed pups. In another report, addition of DMAE or monomethylethanolamine to cultured hepatocytes isolated from choline-deficient rats resulted in the biosynthesis of phosphatidyldimethylethanolamine or phosphatidylmonomethylethanolamine in the place of phosphatidylcholine. Phosphatidyldimethylethanolamine corrected, to a limited extent, the choline-deficient reduction in Very low density lipoprotein (VLDL) secretion. Without VLDL secretion, fat and cholesterol accumulate in the liver, producing liver damage (Oregon State University, 2000).

For Monoethanolamine (MEA), the absorption, distribution and metabolism of topically administered [¹⁴C]-MEA was studied in vivo, using athymic nude mice and human skin grafted onto athymic nude mice (Klain et al, 1985).The results indicated that topical applied MEA penetrates the skin and is widely distributed in the body, radioactivity being detected in all the tissues and organs examined.Radioactivity in expired CO2 was detected 5 min after an intraperitoneal administration of MEA, while no radioactivity in expired air was detected during the first 20 min post-topical application. The substance is readily metabolised in the skin as well as in other organs and tissues in the mouse. Liver is a major site for metabolism, containing over 24% of the applied radioactive dose. Further recovery of the administered radioactive dose was at skin administration site (24.3%), as exhaled CO2 (over 18%), in urine (4.6%), in kidneys (2.5%) and in feces (1.8%). Lungs, brain, and the heart contained 0.55, 0.27, and 0.15% of the dose, respectively. Extensive metabolisation was indicated by appearance of labelled carbon dioxide in skin and hepatic amino acids, proteins and incorporation into phospholipids, and by recovery of over 18% of radioactive dose as 14CO2. Urea, glycine, serine, choline, and uric acid were the urinary metabolites of MEA. Pilgeram described a study where following an intraperitoneal application of 1,2 -¹⁴C-ethanolamine 12 % of the total dose could be recovered within 24 hours. Of these the majority was exhaled in form of CO₂(9.2 %), another 2.84 % were excreted via the urine (Pilgeram, 1953)

Skin penetration of MEA was also studied in vitro (Klain et al, 1985). The results show that percutaneous penetration of MEA was quite slow, with ca. 5% penetrating the skin 50 hours post-application. Ca. 11% was lost from the skin due to evaporation and the bulk of the dose 62% remained in the skin. It should be noted that the in vitro penetration rate of MEA in pig skin was considerably slower than the rate observed in the in vivo experiment likely to be based on differences in species, thickness of the skin, or the presence of the microcirculation system. In a study published by Sun et al. (1996), skin penetration of MEA was tested in an in vitro model with full thickness skin preparations from mice, rats, humans and rabbits. The steady-state penetration rates were determined to be 42.5, 123.1, 73.8, and 7.9 μg/cm²/h for the undiluted dose of [14C]-MEA, while the permeability constants were calculated to be 0.42 x 10-4, 1.21 x 10-4, 0.72 x 10-4, and 0.08 x 10-4cm/hr for rat, mouse, rabbit, and human skin preparations, respectively. Comparing undiluted and water diluted doses of MEA, the results showed that there was generally less skin penetration of the undiluted material than that for the diluted test substance.The results suggest that the potential percutaneous absorption of MEA would be less for humans than it would be for rats, rabbits, and mice.The results indicate that topically applied MEA penetrates the skin, is widely distributed and is extensively metabolised in the body.

SO2 transforms into sulfite after inhalation and exposure leads to a dose-dependent and significant increase of the contents of sulfite in brain, heart, and lung tissues (Meng, 2005).Endogenously generated sulfite resulted in significant systemic exposure in the highly sulfite oxidase-deficient groups. The histological data and results of gross pathological examinations indicate that all organs except the testicles were refractory to theses high concentrations of sulfite (Gunnison, 1987). Following oral administration of 10 or 50 mg SO2/kg (as NaHSO3 mixed with Na235SO3), 70-95% of the 35S was absorbed from the intestine and voided in the urine of mice, rats and monkeys within 24 hours. The majority of the remaining 35S was eliminated via faeces, the rate being species-dependent. Only 2% or less of 35S remained in the carcass after one week (Gunnison&Palmes, 1976; Gibson & Strong, 1973).In conclusion, soluble sulfite substances may be considered to be readily absorbed from the digestive tract of mice and rats; these studies also showed that only small amounts of ingested SO2 are eliminated via exhalation. Given the somewhat variable but high extent of oral absorption (70-95%), it appears most appropriate to conservatively assume 100 % absorption of sulfite substances after ingestion.Sulfites are cleared from the body almost exclusively by oxidation to sulfate. Exposure to sodium dithionite leads to composition products such as hydrogen sulfite which can be absorbed from the rat gastrointestinal tract and is oxidised in vivo to sulfate, principally by hepatic sulfite oxidase (cytochrom-c oxidoreductase). With lesser amounts, it is metabolised by the kidneys, intestines, heart, and lungs. About 70 to 95 % of the radioactivity orally applied hydrogen sulfite dose appeared in rodent and monkey urine within 3 days as sulfate. Only a small fraction (8 - 10%) of the absorbed hydrogen sulfite was eliminated intact (ACGIH, 1991; Gunnison, 1977). Physiologically, sulfite oxidase is involved in the methionine and cysteine metabolism.The endogenous sulfite amount in the body resulting from amino acid degradation is in the range of 0.3 - 0.4 mmol/kg. This is reported to be about 15 - to 130 - fold higher than the estimated value for exogenous sulfite exposure (Institute of Food Technologists and Committee on Public Information, 1976). In conclusion, sodium dithionite is not stable under physiological conditions. The rate of decomposition increases with increasing acidity. Upon contact with moisture, it is oxidised to hydrogen sulfite (HSO3-), sulfite (SO32-) and hydrogen sulfate (HSO4-), and under strongly acidic conditions it may liberate sulfur dioxide. Under anaerobic conditions (such as in the lower gastrointestinal tract), hydrogen sulfite (HSO3-) and thiosulfate (S2O32-) may be formed.Thiosulfate is eliminated mainly unchanged via renal excretion, but some amounts can also be enzymatically oxidized in the liver to sulfate. Under physiologically relevant conditions, the substance readily dissociates in aqueous solution to sulfite and hydrogensulfite anions. Bioaccumulation is not expected because of the strong anionic and hydrophilic nature of the substance, as well as its experimentally verified rapid oxidation in vivo to sulfate with subsequent renal elimination.