(2-hydroxy-3-sulphopropyl)dimethyl[3-[(1-oxododecyl)amino]propyl]ammonium hydroxide

Sediment toxicity

Link to relevant study record(s)

Reference

Endpoint:

sediment toxicity: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 january 2009-11 march 2009

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Despite the lack of analytical monitoring data, it can be reasonably assumed that the test concentrations remained stable over the test duration from the analytical measurements reported in the long-term toxicity test to Daphnia magna performed on a structural analogue substance (Höger, 2013).

Qualifier:

according to guideline

Guideline:

other: OSPARCOM Guidelines (2005) - A Sediment bioassay using an amphipod Corophium sp.

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test solutions were prepared without any organic solvent.
- Controls: five replicates.
- Reference vessels: no reference vessels.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none.

Vehicle:

no

Details on sediment and application:

- Source: collected on 12 january 2009 from a site in the Bay of Suckquoy, Toab in Orkney (adjacent to Corophium collection) .
- Treatment on the laboratory: the sediment was wet-sieved through 0.6 mm mesh to remove larger infaunal organisms and Corophium within the test size range, and allowed to settle in tall polyethylene containers. After setting, the supernatant water was decanted and the sediment stored in a room at approximately 4 +/- 2°C, until required for testing.
- Characterisation: particule size analysis characterised the sediment as well-sorted, fine sand with a silt/clay content of 3.56% by weight. median particle was 151 µm. The organic material content was estimated from weight loss on ignition to be 1.34%.
- Ratio wet/dry weight: immediately before the initiation of the test, the sediment was thoroughly homogenised and a representative sample taken for wet and dry weight determination. The ratio of wet weight to dry weight was subsequently used to convert nominal exposure concentrations on a wet weight basis to nominal exposure concentrations on a dry weight basis. The wet to dry ratio of sediment was found to be of 1.47.

Test organisms (species):

Corophium volutator

Details on test organisms:

TEST ORGANISM
- Common name: Corophium volutator.
- Source: collected on 12 january 2009 from a site in the bay of Suckquoy, Toab in Orkney. Specimens were gently sieved from their native sediment and held in plastic containers (approximately 5L capacity) containing some ambient water, with a small amount of detritus, until tranfer to the laboratory.
- Treatment at the laboratory: on return to the laboratory, the Corophium were transferred in ambient water to polythene tanks of approximately 200 L capacity, gentle aeration was suppplied. The tank were held in a controlled-temperature room at approximately 15 +/- 2°C.
- Age at study initiation: specimens of approximately 5 mm in body length (excluding rostum).

ACCLIMATION
- Acclimation period / acclimation conditions: the stock animals were gradually acclimated from the ambient salinity of less than 5 per thousand to that of undiluted seawater (approximately 35 per thousand) in increments of approximately 10 per thousand per day. Once acclimation is complete, the stock tanks were maintained under semi-static conditions until the initiation of the test. The holding period in the laboratory was 4 days prior to testing. Sufficient detrital material is retained in each tank to provide food and some bottom cover, but not of a density that prevents daily observation of mortality and morbidity. Dead or impaired are removed when observed.

Study type:

laboratory study

Test type:

static

Water media type:

saltwater

Type of sediment:

natural sediment

Limit test:

no

Duration:

10 d

Exposure phase:

total exposure duration

Test temperature:

13.5-15.1°C

pH:

7.89-8.30

Dissolved oxygen:

93-99%

Salinity:

34-39 ‰

Nominal and measured concentrations:

Target wet weight nominal concentrations : 10, 100, 320, 1000 and 10000 mg test item/kg sediment.
Nominal dry weight concentration: 14.78, 146.77, 470.49, 1468.73 and 14692.04 mg test item/kg dw sediment.

Details on test conditions:

TEST SYSTEM
- Test vessel: 1 L capacity glass beakers containing 2 cm depth (approximately 150 ml) of amended sediment and 850 ml of overlying seawater (1 μm filtered ultra violet treated seawater).
- Location /organization: the beakers were assigned positions within the test area, arranged in rows of three to five and spaced to maintain effective separation of different treatments. Each row was covered with a rectangular sheet of perspex perforated with a small hole above the centre of each beaker.
- Aeration : aeration provided and a stream of air bubbles were released at a depth of approximately 6 cm.
- No. of organisms per vessel: 20.
- No. of vessels per concentration (replicates): 3.
- No. of vessels per control (replicates): 5.

TEST MEDIUM / WATER PARAMETERS
- Source : seawater was supplied by pump from Scapa Flow, Orkney. Seawater was UV sterilised and filtered to 1µm before being employed for testing.

TEST SOLUTIONS
- Method: the test solutions were prepared without using of any organic solvent.
- Stock solution: a 1000 mg/L stock solution was prepared in filtered seawater and the resulting mixture was stirred for one hour.
- Controls: treated seawater without test item.
- Evidence of undissolved material (e.g; precipate, surface film, etc): no.

OTHER TEST CONDITIONS
- Photoperiod: constant illumination.
- Light intensity: no data.

Reference substance (positive control):

no

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

> 14 695 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

> 4 408.5 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Duration:

10 d

Dose descriptor:

NOEC

Effect conc.:

14 695 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

10 d

Dose descriptor:

NOEC

Effect conc.:

ca. 4 408.5 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

The test item exhibited a 10 day LC50 value of greater than 14694.78 mg/kg dw to the marine amphipod Corophium volutator in the sediment phase.

The following validity criteria were fulfilled:
- mortality of controls is less than 15% at the end of the test;
- test conditions (pH, temperature) were maintained during the test.

Validity criteria fulfilled:

yes

Conclusions:

The test item exhibited no adverse effects on Corophium volutator up to the nominal concentration of 14692 mg/kg sediment dw.

Executive summary:

The sediment toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study performed with the marine amphidod species Corophium volutator in accordance with OSPARCOM guidelines (2005). The test item exhibited a 10-day LC50 value greater than 14695 mg/kg sediment dw (corresponding to 4408.5 mg active content/kg sediment dw).

Description of key information

The sediment toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study (Hudson, 2009) performed with the marine amphidod species Corophium volutator in accordance with OSPARCOM guidelines (2005). The test item exhibited a 10-day LC50 value greater than 14695 mg/kg sediment dw (corresponding to 4408.5 mg active content/kg sediment dw).

NB. In case the solid content of the test item was not reported in a study report, ECx values were expressed in active content. If relevant, these values were used for the chemical safety assessment (worst-case values).

Key value for chemical safety assessment

EC50 or LC50 for marine water sediment:

4 408.5 mg/kg sediment dw

Additional information

The sediment toxicity of the substance EC 242-893-1 was investigated in a GLP-compliant study (Hudson, 2009) performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 2) and was selected as key study for the endpoint.