Registration Dossier
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EC number: 202-216-2 | CAS number: 93-08-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Short term toxicity of test chemical were studied on the mortality of fish.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24 hrs continuous stirring for achieving test concentrations of 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L ,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.86 cm
- Weight at study initiation (mean and range, SD): average 0.193 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 149.5 mg of CaCO3
- Test temperature:
- 24.1 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.7 mg/l
- Nominal and measured concentrations:
- Nominal concentrations : 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Table 1. Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.3125
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.625 4 4 - - - - - - - - - - - - 1.25 4 4 - - - - - - - - - - - - 2.5 4 4 - - - - - - - - - - - - 5 4 4 - - - - - - 1 1 2 2 2 2 Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.3125
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.625
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
1.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
2.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
5.0
4 4 NS4 NS4 NS4 NS4 NS4 NS4 NS3 NS3 NS2 NS2 NS2 NS2 Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study concludes that the test chemical is toxic to the aquatic environment at a median lethal concentration (LC50) of 5.0 mg/L.
- Executive summary:
The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2. The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.
Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L. The test chemical is toxic to the aquatic environment and it can be classified as aquatic chronic category 2.
Reference
Description of key information
The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2.
The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.
Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5 mg/L
Additional information
The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2. The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.
Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L. The test chemical is toxic to the aquatic environment and it can be classified as aquatic chronic category 2.
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