Dialuminium zinc tetraoxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Mar - 16 Mar 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 1992

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

adopted 2008

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Samples were taken from the control and the 100 mg/L treatment at 0 and 96 h.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: As solubility lies below 100 mg/L, a saturated solution was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: The original animals were obtained from Umweltbundesamt (UBA) Berlin, Germany and used for breeding and production of eggs.
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation: 2 ± 1 cm
- Feeding during test: none

ANIMAL HUSBANDRY
- Culture vessels: polyethylene aquaria
- Culture medium: dechlorinated tap water
- Type and amount of food: warm-water fish food and daphnia, approx. 1 - 2% of body weight per day
- Feeding frequency: three times per day until 24 h before test start
- Medium renewal: twice per week
- Light and temperature: 12 h light/12 h dark, 28 °C

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1.08 mmol/L

Test temperature:

21.5 - 23.2 °C

pH:

7.6 - 8.1

Dissolved oxygen:

7.7 - 8.8 mg/L

Nominal and measured concentrations:

Nominal: control and 100 mg/L
Measured concentrations of zinc at 0 h: control 0.058 mg/L, treatment 0.038 mg/L
Measured concentrations of zinc after 96 h: control 0.025 mg/L, treatment 0.030 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, fill volume: glass, 10 L, 7 L
- Aeration: frequency of bubbles 1/second
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: good quality drinking water
- Total organic carbon: 0.5 mg/L
- Metals (mg/L): Na 5.2, K 2.6, Ca 35.7, Mg 4.6, Al 0.007, Fe/Mn/Sb/As/Pb/Cd/Cr/Cu/Ni/Hg/Se < 0.005
- Pesticides: < LOD
- Chloride: 12 mg/L
- Ca/Mg ratio: 7.76:1
- Conductivity: 223 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: Oxygen concentration and pH were measured every 24 h.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/12 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality and abnormal behaviour were observed every 24 h.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: filtered test solution

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No abnormalities were observed in the control and test vessels.
- Mortality of control: No mortality was observed in the control.
- Effect concentrations exceeding solubility of substance in test medium: yes
- All validity criteria are met.
- Stability of test item solutions: After 96 h 94% of the initial zinc amount were measured, hence the standard in Danio test medium showed sufficient stability for zinc content over the test period.

Description of key information

LC50 (96h) > 100 mg/L for Danio rerio (OECD 203)

Additional information

One study on fish toxicity is available for Zinc aluminium oxide. This key study was performed as a limit test according to OECD Guideline 203, in a static system, using Danio rerio as test organism (2012). As the solubility of the test substance lies below 100 mg/L, a saturated solution was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters. The fish were exposed to the filtered test solution for 96 hours. As no mortalities were observed during the test, the LC50 was determined to be > 100 mg/L. Measured Zn concentrations in control and test solution were in the same range (control: 0.058 - 0.03 mg Zn/L, 100 mg/L: 0.03 - 0.038 mg Zn /L).