Dialuminium zinc tetraoxide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 Mar - 2 May 2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, non-GLP, no analytical concentration control

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was dissolved directly in dilution water. 0.100 g of test item was weighed and added to each vessel. Dilution water was filled up to 1000 mL per test vessel.
- Controls: Control contained dilution water only.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Landesanstalt für Umweltschutz Baden-Württemberg, Griesbachstr. 1, D-76185 Karlsruhe
- Age at study initiation: < 24 h
- Method of breeding: medium M4; breeding in the testing facility under standardised laboratory conditions; breeding was performed in glass beakers (50mL) under the same conditions as in the test
- Feeding during test
- Food type: alga Desmodesmus subspicatus
- Amount: 0.5 - 1.5 mL/50 mL M4, depending on age
- Frequency: daily

ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: alga Desmodesmus subspicatus
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2 mmol/L

Test temperature:

20.0 - 20.5 °C

pH:

7.95 - 8.01

Dissolved oxygen:

8.51 - 8.66 mg/L

Nominal and measured concentrations:

nominal: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: glass beaker, 1000 mL, fill volume: 1000mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 200 mL test solution per Daphnia

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to ISO 6341
- Alkalinity: 200 mg/L CaCO3
- Ca/Mg ratio: 4:1
- Conductivity: 0.646 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, dissolved oxygen and temperature determined at the start and at the end

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment pf pH
- Photoperiod: 16 h light: 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): number of immobilised daphnids, including discoloration, anomalies after 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: up to 100 mg/L
- Results used to determine the conditions for the definitive study: no immobilisation occurred

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: only NOEC reported

Details on results:

- No immobilisation occurred during the study
- No visible abnormalities were observed in the control or treatment groups
- Validity criteria are fulfilled

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 24 h 1.53 mg/L and 48 h 1.28 mg/L

Description of key information

EC50 (48h) > 100 mg/L for Daphnia magna (OECD 202)

Additional information

One study on the short-term toxicity of Zinc aluminium oxide to aquatic invertebrates is available. This key study was performed as a limit test according to OECD Guideline 202, in a static system, using Daphnia magna as test organism (2005).The test solutions were prepared by direct addition of the item in dilution water, and were not filtered. The daphnids were exposed to a loading of 100 mg/L during 48 hours. No mortalities were observed and a NOEC of 100 mg/L (nominal) is reported. The EC50 is thus > 100 mg/L (nominal). Analytical measurements of zinc or aluminium were not performed.