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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopulegol
EC Number:
201-940-6
EC Name:
Isopulegol
Cas Number:
89-79-2
Molecular formula:
C10H18O
IUPAC Name:
isopulegol
Details on test material:
- Name of test substance: L-Isopulegol
- Test substance No.: 10/0358-2
- purity: 99.95 corr. area %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
- Age (on study day 0): A three day old preculture, prepared in dilution water, was used as inoculum.
- Supplier: Sammlung von Algenkulturen (SAG); Pflanzenphysiologisches Institut der Universität Göttingen; Nikolausberger Weg 18, D-37073 Göttingen

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: Sterile headspace flasks, volume: 119 ml, aluminium tops with PTFE seals.
- Initial cells density: 7.085 x 10^3 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: Nutrient medium Z according to LüTTGE et al. (1994)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 24h/d light
- Light intensity and quality: Approximately 60 - 120 μE · m-2 · s-1 (Fluorescent tubes, OSRAM L 36 W/11-865, cool daylight)
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
50.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
35.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes

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