1-Propanethiol, 3-(trimethoxysilyl)-, adduct with 5-isocyanato-1-(isocyanatomethyl)-1,3,3-trimethylcyclohexane (1:1)

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-05-20

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: ICCVAM Test Method Evaluation Report: Appendix G, ICCVAM recommended HET-CAM Method Protocol (Nov. 2006)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Background Review Document; „Current Status of In Vitro Test Methods for Identify-ing Ocular Corrosives and Severe Irritants: Hen’s Egg Test - Chorioallantoic Mem-brane Test Method (March 2006, NIH Publication No. 06-4515)

Deviations:

no

Principles of method if other than guideline:

not applicable

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,3,12,12-tetramethoxy-2,13-dioxa-7,8-dithia-3,12-disilatetradecane; 5-(7,7-dimethoxy-2-oxo-8-oxa-3-thia-1-aza-7-silanonan-1-yl)-1-[(7,7-dimethoxy-2-oxo-8-oxa-3-thia-1-aza-7-silanonan-1-yl)methyl]-1,3,3-trimethylcyclohexane; [3-({[(3-isocyanato-3,5,5-trimethylcyclohexyl)methyl]carbamoyl}sulfanyl)propyl]trimethoxysilane; [3-({[(5-isocyanato-1,3,3-trimethylcyclohexyl)methyl]carbamoyl}sulfanyl)propyl]trimethoxysilane

EC Number:

700-534-0

Cas Number:

117172-56-2

Molecular formula:

Not applicable: UVCB substance, see section 1.2 and 1.4. The UVCB substance consits of differnt products consiting of differnt isomers: Product I and Product II cannot analytically be distinguished and moreover, Product I-III are expected to consist of isomers that can neither analytically be identified nor quantified.

IUPAC Name:

3,3,12,12-tetramethoxy-2,13-dioxa-7,8-dithia-3,12-disilatetradecane; 5-(7,7-dimethoxy-2-oxo-8-oxa-3-thia-1-aza-7-silanonan-1-yl)-1-[(7,7-dimethoxy-2-oxo-8-oxa-3-thia-1-aza-7-silanonan-1-yl)methyl]-1,3,3-trimethylcyclohexane; [3-({[(3-isocyanato-3,5,5-trimethylcyclohexyl)methyl]carbamoyl}sulfanyl)propyl]trimethoxysilane; [3-({[(5-isocyanato-1,3,3-trimethylcyclohexyl)methyl]carbamoyl}sulfanyl)propyl]trimethoxysilane

Constituent 2

Reference substance name:

Reaction products of 3-(trimethoxysilyl)propane-1-thiol and 5-isocyanato-1-(isocyanatomethyl)-1,3,3-trimethylcyclohexane (1:1)

IUPAC Name:

Reaction products of 3-(trimethoxysilyl)propane-1-thiol and 5-isocyanato-1-(isocyanatomethyl)-1,3,3-trimethylcyclohexane (1:1)

Details on test material:

- Name of test material: Intermediate 36
- Colour: light yellow
- Physical state: liquid
- Analytical purity: >99%

Test animals / tissue source

Species:

other: chicken eggs

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: 3 eggs

Duration of treatment / exposure:

not applicable

Observation period (in vivo):

5 minutes (301 seconds)

Number of animals or in vitro replicates:

Intermediate 36 was tested using 6 eggs, for each of the 2 positive controls and for the negative control, 3 eggs were used.

Results and discussion

In vivo

Other effects:

not applicable

Any other information on results incl. tables

Experimental Observations

Test Group                      Time until Haemorrhage                     Time until Lysis              Time until Coagulation

seconds                                    seconds                            seconds

Negative Control :                         -                                                -                                      -

Negative Control:                     -                                                - -

Negative Control - - -

Positive Control (0.1 N NaOH) 9, 31, 22

Positive Control (0.1 N NaOH) 8, 33, 19

Positive Control (0.1 N NaOH) 8, 34, 20

Positive Control (1% SDS) 14, 54, -

Positive Control (1% SDS) 12, 58, -

Positive Control (1% SDS) 15, 59, -

Intermediate 36: - - -

Intermediate 36: - - -

Intermediate 36 : - - -

Intermediate 36: - - -

Intermediate 36: - - -

Intermediate 36: - - -

- : no reaction observed after 301 seconds.

The positive controls induced a severe irritation on the blood vessels, the irritation score for the positive controls lay within the demanded range. The negative control showed no irritation. The test item was tested pure. A mean irritation score of 0.0 was calculated, corresponding to a classification as “not irritant”. No observations were made which might cause doubts concerning the validity of the study outcome. The test is considered valid.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: other:

Conclusions:

A mean irritation score of 0.0 was calculated, corresponding to a classification as “not irritant”. No observations were made which might cause doubts concerning the validity of the study outcome.

Executive summary:

This in vitro study was performed to assess the irritating potential of Intermediate 36 by detection of damages in blood vessels under the chorionallantoic membrane of incubated chicken eggs (nine days old). Observation time was 5 minutes at room temperature. Physiological sodium chloride solution was used as negative control, sodium dodecyl sulfate (1% solution) and sodium hydroxide (0.1 N solution) were used as positive controls. The positive controls induced a severe irritation on the blood vessels, the irritation score for the positive controls lay within the demanded range. The negative control showed no irritation. The test item was tested pure. A mean irritation score of 0.0 was calculated, correspond-ing to a classification as "not irritant". No observations were made which might cause doubts concerning the validity of the study outcome. The test is considered valid.