O,O-diisopropyl hydrogen dithiophosphate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

O,O-diisopropyl hydrogen dithiophosphate

EC Number:

203-503-5

EC Name:

O,O-diisopropyl hydrogen dithiophosphate

Cas Number:

107-56-2

Molecular formula:

C6H15O2PS2

IUPAC Name:

O,O-bis(propan-2-yl) sulfanylphosphonothioate

Constituent 2

Reference substance name:

2-ethoxyethylamine

EC Number:

203-801-5

EC Name:

2-ethoxyethylamine

Cas Number:

110-76-9

IUPAC Name:

2-ethoxyethanamine

Test material form:

other: liquid

Details on test material:

- Analytical purity: 98 % w/w

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

- Concentrations:(nominial) 0, 2, 5, 10, 20, 50, 100 and 200 mg/l

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Skeletonema costatum

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): DHI

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

18.5 ºC

pH:

8.1

Salinity:

28 ‰

Nominal and measured concentrations:

Nominal concentrations: 0, 2, 5, 10, 20, 50, 100 and 200 mg/l

Details on test conditions:

- Test vessels: 300 ml glass flasks with approx. 100 ml of test soulution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 19.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50: 1.17 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50( h) of O,O-diisopropyl hydrogen dithiophosphate, CAS No.107-56-2, is 19.3 mg/l for Skeletonema costatum