O,O-diethyl hydrogen phosphorodithioate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted in mice comparable to the method described in OECD guideline 401.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not applicable

Principles of method if other than guideline:

Mice were starved overight before exposure. Test substance was prepared as a 20% emulsion in Tween-80 (10% aqueous dilution) and administered at a maximum dose volume of 20 mL/kg bw. Observation period was 14 days.

GLP compliance:

not specified

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

O,O-diethyl hydrogen phosphorodithioate

EC Number:

206-055-9

EC Name:

O,O-diethyl hydrogen phosphorodithioate

Cas Number:

298-06-6

Molecular formula:

C4H11O2PS2

IUPAC Name:

O,O-diethyl sulfanylphosphonothioate

Details on test material:

- Name of test material (as cited in study report): EP1, O,O-diethyl-ditbiophosphoric acid

Test animals

Species:

mouse

Strain:

CD-1

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 16 - 22 g
- Fasting period before study: overnight

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Tween-80 (10% aqueous dilution)

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 20% v/v in Tween-80
- Amount of vehicle (if gavage): maximum dose of 20 mL/kg

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg

Doses:

0, 0.64, 1.0, 1.6, 2.5, 4.0 mL/kg bw

No. of animals per sex per dose:

5/sex/dose

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: body weight

Statistics:

Not applicable.

Results and discussion

Preliminary study:

Results of a preliminary range finding tests indicated that the median lethal oral dose was in the region of 1 - 1.6 mL/kg bw.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1.2 mL/kg bw

Based on:

test mat.

95% CL:

1.1 - 1.4

Mortality:

Death occured between 2 and 92 hours of treatment.

Clinical signs:

other: Lethargy was observed in treated animals within a few hours of dosing.

Gross pathology:

Darkening of the liver. Animals treated at 4 mL/kg showed ulceration of the stomach wall and intestine together with bleaching of parts of the liver.

Other findings:

Not applicable

Any other information on results incl. tables

No other information.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: OECD GHS

Conclusions:

The acute median lethal oral dose (LD50) and its 95% confidence limits to mice of EP1 were calculated to be: 1.2 (1.1 - 1.4) mL/kg bw., corresponding to 0.24 (0.22 - 0.28) g/kg bw .

Executive summary:

An acute oral toxicity study was performed in mice on EP1.

EP1 was prepared as a 20% v/v emulsion in Tween-80 (10% aqueous solution) and administered at a maximum dosage volume of 20 mL/kg. Mice treated at Tween-80 (10%) in water (20 mL/kg) served as control.

During the 14 -days observation period, record was kept of all mortalities and signs of toxicity. All mice were examined macroscopically to identify target organs.

Autopsy revealed darkening of the liver and animals treated at 4 mL/kg (0.8 g/kg bw) showed ulceration of the stomach wall and intestine togeher with bleaching of parts of the liver.

The acute median lethal oral dose (LD50) of EP1 were calculated to be 1.2 mL/kg bw (0.24 g/kg bw).