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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Antibactcerial effect of test material on Pseudomonas putida
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Total exposure duration:
16 h
Test temperature:
26 degC
Details on test conditions:
The carrier of fluoranthene and its metabolites was a recommended mineral medium, composed for the test as follows (mg/l): NaNO3 ¼ 500; K2HPO4 ¼ 120; KH2PO4 ¼ 60; D(+) glucose monohydrate ¼ 2000; MgSO4 7H2O ¼ 200 and Iron(III) citrate ¼ 0.5. The pH was adjusted to 7.40.3.
In mineral medium; bacterial cultures incubated with title comp. in 26 deg C for ca. 16 h;turbidity of samples measured with spectrophotometer at 436 nm
Reference substance (positive control):
not specified
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
223 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Conclusions:
The effective concentration (EC50) value of test material in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was found to be 223 mg/L.
Executive summary:

The effect of test material was The effective concentration (EC50) value of test material in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was found to be 223 mg/L.

Description of key information

Toxicity to microorganism:

The effect of test material was evaluated on Pseudomonas putida . The effective concentration (EC50) value of test material in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was found to be 223 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
223 mg/L

Additional information

Toxicity to microorganism:

Effect of test material was evaluated on various microorganisms , the effect of test material was evaluated on Pseudomonas putida . The effective concentration (EC50) value of test material in Pseudomonas putida in a 16 hr study on the basis of growth inhibition effect was found to be 223 mg/L.

In another peer reviewed journal , toxicity study of micro-organism to the test substance was conducted usingPhotobacterium phosphoreum, strain NRRL-B-11177 (also referred to asVibrio fischerii, strain NRRL-B-11177). When test bacteria was exposed to the test substance test material with exposure duration of 5, 15 and 30 mins, reduction in light output was observed. The EC50 value during 30 mins exposure period was found to be 542 mg/l.