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EC number: 252-772-5 | CAS number: 35869-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- occlusive dressing, 24 hours exposure duration, only 24- and 72-hour scoring available, 72-hour observation period
- GLP compliance:
- no
Test material
- Reference substance name:
- N,N'-(2-chloro-1,4-phenylene)bis[4-[(4-chloro-2-nitrophenyl)azo]-3-hydroxynaphthalene-2-carboxamide]
- EC Number:
- 252-772-5
- EC Name:
- N,N'-(2-chloro-1,4-phenylene)bis[4-[(4-chloro-2-nitrophenyl)azo]-3-hydroxynaphthalene-2-carboxamide]
- Cas Number:
- 35869-64-8
- Molecular formula:
- C40H23Cl3N8O8
- IUPAC Name:
- N,N'-(2-chloro-1,4-phenylene)bis{4-[(4-chloro-2-nitrophenyl)diazenyl]-3-hydroxy-2-naphthamide}
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Physical state: brown powder
Constituent 1
- Specific details on test material used for the study:
- - Physical state: brown powder
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Porton strain bred on the premises
- Age at study initiation: 3-4 months
- Weight at study initiation: ca. 2.23 kg for males and 2.55 kg for females
- Housing: caged singly in an experimental room
- Diet (e.g. ad libitum): commercial irradiated diet (Styles-Oxoid); ad libitum
- Water (e.g. ad libitum): sterile filtered drinking water; ad libitum
- Acclimation period: one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 1°C
- Humidity (%): 50-70%
- Photoperiod (hrs dark / hrs light): 10/14
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- other: shaved or abraded (only data from the shaved skin sites are presented here, since they are suitable for assessment)
- Vehicle:
- other: 50% aqueous solution of polyethylene glycol
- Controls:
- other: untreated skin sites of the same animals
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g
- Concentration (if solution): 10 g of the test compound was mixed with 20 ml of a 50% aqueous solution of polyethylene glycol to make a solution of 25ml, 1.25ml of which was applied to each test site. - Duration of treatment / exposure:
- 24 hours
- Observation period:
- 72 hours
- Number of animals:
- 6 (3 males and 3 females)
- Details on study design:
- TEST SITE
- Area of exposure: at least 10% of the total body surface (the whole back) was shaved 24 hours before treatment). The shaven skin on the rightt side was slightly scarified immediately before treatment. The abrasions were sufficiently deep to penetrate the stratum corneum but not to damage the dermis. The left hand site remained intact.
- % coverage: a 2.5 cm square gauze pad was used
- Type of wrap if used: the pad was covered with aluminium foil secured with Sleek adhesive tape. The test sites were then enclosed by a 6" wide Coban self adhesive bandage, the edges of which were fixed to the skin by strips of Sleek in order to retain the test substance in close contact with the skin.
REMOVAL OF TEST SUBSTANCE
- Time after start of exposure: the gauze patches were removed 24 hours after the application
SCORING SYSTEM: comparable to the OECD scoring system (only 24- and 72-hour scores were determined)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- Mean of all tested animals
- Time point:
- other: 24-72-hours
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Remarks on result:
- other: 48-hour scoring not performed
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- Mean of all tested animals
- Time point:
- other: 24-72-hours
- Score:
- 0.5
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: 48-hour scoring not performed
- Irritant / corrosive response data:
- No erythema formation was observed, neither on intact skin sites nor on the abraded ones. Edema was observed in all but one animals after 24 hours on intact skin sites (one animal with edema score 2 and 4 animals each with edema score 1), and in 4/6 animals at abraded skin sites (one animal with edama score 2 and 3 animals each with edema score 1). The edema formation was completely reversible within 72 hours. It could therefore be assumed that the test substance is not irritating according to EU-CLP.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
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