17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

EC Number:

259-700-1

EC Name:

17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

Cas Number:

55542-26-2

Molecular formula:

C22H30O3

IUPAC Name:

(1S,3aS,3bR,9aR,9bS,11aS)-1-hydroxy-1-(3-hydroxyprop-1-yn-1-yl)-9a,11a-dimethyl-1H,2H,3H,3aH,3bH,4H,5H,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-one

Details on test material:

- Name of test material (as cited in study report): Hydroxypropinol
- Analytical purity: 98.9%
- Lot/batch No.: 59002501

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

54.89 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Table 2: Cumulative immobilization of Daphnia magna after exposure to ZK

Concentration of	Total no. of	No. of immobilized Daphnia
ZK4829	Daphnia		24 h			48h
		1	2	3	4	1	2	3	4
Control (dilution water)	20	0	0	0	0	0	0	0	0
54,89 mg/L	20	0	0	1	0	0	0	1	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

It can be concluded that the EC50/48 hours of ZK4829 to Daphnia magna is higher than
saturated solution (54.89 mg/L).

Executive summary:

20 juvenile Daphnia magna were used for the saturated test solution of Hydroxypropinol and 20 for the dilution water control. The Daphnia were exposed for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours.

The organic carbon concentration of the test solution was analyzed with a TOC analyzer at the

start of exposure and 48 hours thereafter. The test item concentration was calculated on the

basis of the molecular formula. The mean concentration was calculated with 54.89 mg/L.

The test temperature was in the range of 20 to 21 0 C, the pH ranged between 7.02 and 7.68,

and the oxygen concentration between 7.14 and 8.93 mg/L. The light/dark rhythm was adjusted

to 16 hours/8 hours.

The EC50/48 hours of Hydroxypropinol to Daphnia magna is expected to be high er than a saturated

solution (54.89 mg/L, as determined by DOC analysis).