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EC number: 259-700-1 | CAS number: 55542-26-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline under GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- - Name of test material (as cited in study report): Hydroxypropinol
- Analytical purity: 98.9%
- Lot/batch No.: 59002501
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 57 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Table 1: Biomass, growth rate and inhibition of biomass and growth rate after 72 hours exposure time
Concentration of Test Substance (mg/L) | Area below the growth curves | Percentage inhibition of the growth | Average specific growth rates | Percentage inhibition of the growth rate |
A | Ai | µ | µi | |
0 | 692447 | 0 | 1,4367 | 0 |
3,8 | 726695 | -4,95 | 1,4472 | -0,73 |
7,6 | 741762 | -7,12 | 1,4723 | -2,48 |
15,3 | 719036 | -3,84 | 1,4552 | -1,28 |
30,5 | 648612 | 6,33 | 1,4435 | -0,47 |
58,2 | 103587 | 85,04 | 0,6457 | 55,06 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Hydroxypropinol produced inhibition effects on the growth of Senedesmus subspicatus ≥ 30.5 mg/L.
- Executive summary:
The test item Hydroxypropinol was incubated in an aqueous solution including nutrients with an algae
population of Scenedesmus subspicatus for a test duration of approximately 72 hours.
For the preparation of the test solutions a suspension with a nominal loading of 100 mg/L
was stirred for 24 hours. The resulting solution served as the highest concentration (saturated solution). It
was further diluted for concentration levels of 1 :2, 1 :4, 1:8 and 1 :16 with nutrient solution.
The organic carbon concentration of the saturated solution was analysed with a DOC analyzer
at the start and end of the incubation. The test item concentration was calculated on the basis
of the molecular formula. The mean measured concentration of the saturated stock solution
was determined with 61.1 mg/L. The further test concentrations were extrapolated trom the
result of the DDC-analysis and are shown in the table below.
The algae were exposed to each concentration in triplicate. Six vessels were prepared for the
control. The algae were incubated on a shaker under continuous light and a temperature of 22.5 - 24.8 °C.
As a parameter for the growth of the algae population, the cell density of the test and control
solutions were measured via Chlorophyll-a-f1uorescence, excitation at 435 nm, emission
685 nm. Out of the f1uorescence va lues cell density values were calculated according to a
calibration curve.
The increase of biomass and the growth rate were calculated on the basis of the cell density.
The calculated biomass and growth rate of each concentration were compared to those of the
controls, and the inhibition was calculated.
Hydroxypropinol had a concentration-depended inhibitory effect on the growth of Scenedesmus
subspicatus. The EbC50 (biomass) was 45.0 mg/L [95% confidence limits: 42.8 - 47.2], the ErC50 (growth rate) was 57.0 mg/L [95% confidence limits: 55.1 - 58.9] on the basis of measured concentrations.
Consequently, Hydroxypropinol is slightly toxic to the green algae Scenedesmus subspicatus.
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