Alkanes, C8-9-iso-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July - September 1961

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. Limited documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Report date:

1961

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Study was performed before actual guideline was established.

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Hydrocarbons, C7-C9, isoalkanes

EC Number:

921-728-3

IUPAC Name:

Hydrocarbons, C7-C9, isoalkanes

Details on test material:

- Name of test material (as cited in study report): MRD-61-33 (ISOPAR C)
- Physical state: colorless liquid
- Substance type: colorless liquid
- Analytical purity: 100% pure commercial product
- Other: aromatic odor

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 101 - 132 g
- Fasting period before study: Food was withheld from the animals for a period of 3 to 4 hours prior to dosage.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 0.1, 1.0 or 10 % volume/volume solution in corn oil (Mazola)

Doses:

31.6; 100; 316; 1000; 3160; and 10,000 µL/kg body weight

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations (mortality and toxic effects): immediately after administration; at 1, 4 and 24 hours; and once daily thereafter for a total of 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: body weight, pathology (brain, liver, kidney), blood levels were collected by exsanguination

Statistics:

no data

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 10 mL/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 7 100 - 7 800 mg/kg bw

Remarks on result:

other: recalculated values based on the LD50 of 10 mL/kg bw; the range is due to the range of density (0.71-0.78 g/cm3)

Mortality:

The test material produced no deaths at any dosage level tested.

Gross pathology:

There were no pathological findings in any animal at autopsy following sacrifice.

Other findings:

The animals at all dosage levels seemed normal in appearance and behaviour following intubation and daily during the 14 -day observation period.

Any other information on results incl. tables

The acute oral LD50 of MRD-61-33 for male albino rats is therefore greater than 10 mL/kg bw corresponding to 7100 - 7800 mg/kg bw.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: CLP

Conclusions:

Under the conditions of this study the test substance, hydrocarbons, C7-C9, isoalkanes, does not need to be classified.

Executive summary:

Under the conditions of this study the test substance, C7-C9, isoalkanes, does not need not to be classified.