(2-methoxyethyl)benzene

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

for read across

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Qualifier:

according to guideline

Guideline:

other: Scenedesmus cell proliferation inhibition test, DIN 38412 part 9, Determination of the inhibitory effect of water constituents green algae

Qualifier:

according to guideline

Guideline:

other:

Version / remarks:

The objctive of the study was to determine effective concentration of test chemical on aquatic algae.

Principles of method if other than guideline:

WoE report is based on two toxicity studies on aquatic algae of the test chemical :
WoE-1 & WoE- 2

GLP compliance:

not specified

Analytical monitoring:

no

Test organisms (species):

other: 2: Scenedesmus subspicatus

Details on test organisms:

2:
- Common name: green algae
- Source (laboratory, culture collection): BASF AG Ludwigshafen

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

490 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 2

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

790 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

162 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 3

Validity criteria fulfilled:

not specified

Conclusions:

Study was conducted to determine effect of test chemical on Scenedesmus subspicatus. For the test chemical, based on effect on growth rate of the test organism green algae, the 72 hr EC10, EC50 and EC90 was determined to be 300,490 and 790 mgIL respectively and 96 h EC50 was 162 mg/l.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae of the test chemical .The studies are as mentioned below:

In first study,toxicity to green algae was carried out according to Scenedesmus cell proliferation inhibition test, DIN 38412 part 9, Determination of the inhibitory effect of water constituents green algae

out for assessing the effect of the test chemical. The study was conducted for 72 hrs on test organism Scenedesmus subspicatus. Thus, based on effect on growth rate of the test organism green algae, the 72 hr EC10, EC50 and EC90 was determined to be 300,490 and 790 mgIL respectively and therefore chemical can be considered as non-toxic to aquatic algae and can be considered to be ‘’not classified’’ as per CLP classification criteria.

In last study, toxicity to green algae was carried out for assessing the effect of the test chemical. The study was conducted for 96hrs. On the basis of growth rate, the median effect concentration (EC50) was determined to be 162 mg/l. Thus, based on the growth rate effect with EC50 value , chemical can be considered as non-toxic to aquatic algae and can be considered to be ‘’not classified’’ as per CLP classification criteria.

Based on the EC50 values , it can be concluded that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Study was conducted to determine effect of test chemical on Scenedesmus subspicatus. For the test chemical, based on effect on growth rate of the test organism green algae, the 72 hr EC10, EC50 and EC90 was determined to be 300,490 and 790 mgIL respectively and 96 h EC50 was 162 mg/l.

Key value for chemical safety assessment

EC50 for freshwater algae:

490 mg/L

Additional information

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae of the test chemical .The studies are as mentioned below:

In first study,toxicity to green algae was carried out according to Scenedesmus cell proliferation inhibition test, DIN 38412 part 9, Determination of the inhibitory effect of water constituents green algae

out for assessing the effect of the test chemical. The study was conducted for 72 hrs on test organism Scenedesmus subspicatus. Thus, based on effect on growth rate of the test organism green algae, the 72 hr EC10, EC50 and EC90 was determined to be 300,490 and 790 mgIL respectively and therefore chemical can be considered as non-toxic to aquatic algae and can be considered to be ‘’not classified’’ as per CLP classification criteria.

In last study, toxicity to green algae was carried out for assessing the effect of the test chemical. The study was conducted for 96hrs. On the basis of growth rate, the median effect concentration (EC50) was determined to be 162 mg/l. Thus, based on the growth rate effect with EC50 value , chemical can be considered as non-toxic to aquatic algae and can be considered to be ‘’not classified’’ as per CLP classification criteria.

Based on the EC50 values , it can be concluded that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.