[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards (without detailed documentation)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

5 mice per sex per dose were administered a single dose of the test substance via intraperitoneal injection. Clinical signs and body weight were monitored and necropsy was carried out at termination or after death if an animal died during the study. Observation period was until day 7 following treatment.

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

EC Number:

249-125-4

EC Name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Cas Number:

28654-73-1

Molecular formula:

C47 H49 Cu N11

IUPAC Name:

Copper,[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyanine-C,C,C-trimethanaminato(2-)-κN29,κN30,κN31,κN32]-

Details on test material:

- Physical state: solid
- Analytical purity: ca. 93%

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain: NMRI-Ivanovas mice
- Weight at study initiation (mean): 27.2 g (males); 19.6 g (females)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

aqueous suspension in 0.5% CMC-solution

Details on exposure:

test substance was applied as a 35% suspension in 0.5% CMC solution (ca. 28.5 mL/kg bw)

Doses:

10000 mg/kg bw

No. of animals per sex per dose:

5 mice

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations after application: day 0 (3 and 20 min and 3 hours), day 1, 2, 5 and 6
- Frequency of weighing: before application and after 2 and 7 days
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed.

Clinical signs:

3 min after application: dyspnoea, tremor and hunched body posture
20 min after application: dyspnoea
3 h and on day 1 after application: hunched body posture, slight apathy
2 days after application: males: no clinical signs were observed; females: dyspnoea, loss in body weight
5 and 6 days after application: no clinical signs were observed

Body weight:

There was no test item-related effect on the body weight
Mean weights: males: 27 g (day 0), 28 g (day 2) and 30 g (day 7); females: 20 g (day 0), 19 g (day 2) and 21 g (day 7)

Gross pathology:

Intraabdominal substance residues, sporadic adhesions