Benzothiazole

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1998

Reference 2

Reference Type:

secondary source

Title:

Chemical Risk Information Platform- Physical Chemical data

Author:

NITE

Year:

2009

Bibliographic source:

http://www.safe.nite.go.jp

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Benzothiazole

EC Number:

202-396-2

EC Name:

Benzothiazole

Cas Number:

95-16-9

Molecular formula:

C7H5NS

IUPAC Name:

benzothiazole

Details on test material:

- Name of test material (as cited in study report): benzothiazole
- Analytical purity: 98.1 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

- Controls: yes (dilution water)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20.4 °C

pH:

8.1 - 8.3

Dissolved oxygen:

8.2 - 8.4 mg/L (48 h)

Nominal and measured concentrations:

nominal: 10, 18, 32, 56 and 100 mg/L
measured: 6.9, 13, 23, 41 and 73 mg/L (geometric mean of initial and end concentration)

Details on test conditions:

TEST SYSTEM
- Test volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: de-chlorinated tap water

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

19 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 16 - 23

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

6.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- EC50 (48h) = 0.62 mg/L

Any other information on results incl. tables

The lose of test substance throughout the test was approx. 36%.

Applicant's summary and conclusion

Executive summary:

MOE (1997):

Japanese Ministry of the Environment conducted a test on acute toxicity to Daphnia magna according to OECD TG 202. After an exposure period of 48 hours a measured EC50 of 19 mg/L based on mobility was found.