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EC number: 203-612-8 | CAS number: 108-74-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 May 2007 to 26 May 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was conducted in accordance with international guidelines in a GLP testing facility.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Modified to marine conditions (OSPAR/PARCOM)
- Qualifier:
- according to guideline
- Guideline:
- other: OSPAR / PARCOM Protocols on methods for the testing of chemicals used in the offshore industry 2006
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP compliance statement only
- Analytical monitoring:
- no
- Details on sampling:
- N/A
- Details on test solutions:
- The behaviour of the test item in seawater was examined according to the method described in the Harmonised Offshore Chemical Notification Format (OSPAR 1995). A nominal 1000 mg/l solution was prepared in dilution water, shaken vigorously and allowed to stand for four hours.A clear colourless solution was obtained. For each nominal concentration the required amount of homogenised sample was added to 10 litres of dilution water.
- Test organisms (species):
- other: Scophthalmus maximus
- Details on test organisms:
- TEST ORGANISMTest Species: Turbot (Scopthalmus maximus)Source: Manx Mariculture, Derbyhaven, Castletown, Isle of Man IM9 1TUDate of Arrival at Laboratory: 07 Febraury 2007Number Received: 150Mortality in Stock after 48 hour Settling Period: 0 % during acclimation periodAccclimation Period: 07 February 2007 to 22 May 2007Acclimation Conditions: Temperature: 13.5 to 14.5 oCDissolved Oxygen: >92 % ASVPhotoperiod: 16 hours light and 8 hours darkFeeding: The Turbot were fed daily using Le Gouessant Turbot Label Rouge at a rate of approximately 2 % body weight per day until 24 hours before test initiation. The Turbot are not fed for the duration of the study.At the end of the test a sample of 10 turbot were weighed and measured.Mean length: 56.2 mmMean weight: 2.41 g
- Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- N/A
- Test temperature:
- Refer to Table 1.
- pH:
- Refer to Table 1.
- Dissolved oxygen:
- Refer to Table 1.
- Salinity:
- Refer to Table 1.
- Nominal and measured concentrations:
- Nominal Concentrations: 1.908 mg/L (4.5 mg/L Amieton M33) with an untreated control (limit test)No analytical monitoring
- Details on test conditions:
- TEST SYSTEMTest Volume: 10 litres.Test Vessel: 12 litre volume aquaria.Number of replicates: 1 replicate at each control and test concentration.Test Organisms: 7 turbot were placed in each of the control and test vessels.Replacement Regime: Semi-static, test solutions replaced after 48 hours, the remaining animals being transferred to freshly prepared test solutions.Test Conditions: The test vessels were maintained at 15.0 ± 2.0oCWater Quality Measurements: The pH (to 0.1), dissolved oxygen (to 1 % ASV), temperature (to 0.5oC) and salinity (‰) were measured. Water quality determinations for each test and control solution were carried out immediately prior to initiating the test, after 24 hours, before and after the 48 hour change of test solutions, after 72 hours and at the end of the 96 hour exposure period.Observation Frequency: Observations and records of mortalities were made after 24, 48, 72 and 96 hours.Calculation and Expression of Results: Cumulative mortalities were calculated for each test concentration and control.
- Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.908 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: N/A
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.908 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: N/A
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.908 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: N/A
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.908 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: N/A
- Details on results:
- No abnormal behaviour was noted during the study.
- Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- No mortalities were observed in the control or test treatments, therefore no statistical analysis was necessary.
- Sublethal observations / clinical signs:
Table 1. Water Quality Parameters during the Study
a) Summary of pH values
Exposure period (hours)
Concentration (mg/L)
Control
1.908
0
8.2
8.3
24
8.2
8.2
48
8.2
8.2
8.2
8.3
72
8.3
8.2
96
8.2
8.2
pH of stock tank at test initiation: 8.2
b) Summary of dissolved oxygen values (% ASV)
Exposure period (hours)
Concentration (mg/L)
Control
1.908
0
97
97
24
99
98
48
99
97
96
97
72
99
98
96
97
96
Dissolved oxygen of stock tank at test initiation: 99 % ASV
c) Summary of temperature values (oC)
Exposure period (hours)
Concentration (mg/L)
Control
1.908
0
13.5
13.5
24
14.0
14.0
48
14.0
14.0
13.5
13.5
72
14.0
14.0
96
14.0
14.0
Temperature of stock tank at test initiation: 14.0oC
d) Summary of salinity (‰)
Exposure period (hours)
Concentration (mg/L)
Control
1.908
0
33
33
24
33
33
48
33
33
33
33
72
33
33
96
34
34
Salinity of stock tank at test initiation: 36 ‰
Table 2. Cumulative % Mortalities
Exposure period (hours)
Concentration (mg/L)
Control
1.908
0
0
0
24
0
0
48
0
0
72
0
0
96
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LC50 value of hexahydro-1,3,5-trimethyl-1,3,5-triazine to Turbot was estimated to be greater than 1.908 mg/L. The highest no observed effect concentration (NOEC) after 96 hours was 1.908 mg/L.
- Executive summary:
Substance: 42.4 % hexahydro-1,3,5 -trimethyl-1,3,5 -triazine in water
Chemex Reference: Sample: ECO020727; Study: ENV8044
Test Species: Turbot (Scophthalmus maximus)
Test Type: Acute toxicity: 96 hour LC50
Test Conditions: OSPAR/PARCOM Protocols on Methods for the Testing of Chemicals used in the Offshore Industry 2006.
OECD 203: Fish Acute Toxicity Test.
Test Period: 22 May 2007 to 26 May 2007
Testing Laboratory: Chemex Environmental International Limited, Unit J, Broad Lane Industrial Estate, Cottenham, Cambridge CB24 8SW, UK.
Results:
Period of Exposure (hours)
LC50 (mg/L hexahydr-1,3,5 -trimethyl-1,3,5 -triazine)
24
> 1.908
48
> 1.908
72
> 1.908
96
> 1.908
96 hour NOEC
1.908 mg/L
96 hour 100 % mortality
Not determined
Reference
Description of key information
96 h LC50 (S. maximus) = >1.908 mg/L, OSPAR/PARCOM, Sacker (2007)
Key value for chemical safety assessment
Marine water fish
Marine water fish
- Effect concentration:
- 1.908 mg/L
Additional information
A single key study was submitted for this endpoint, conducted in accordance with PARCOM guidelines. The 96h LC50 to S. maximus was estimated to be >1.908 mg/L (Sacker, 2007).
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