2-[2-(dimethylamino)ethoxy]ethanol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental: 10 Apr 1987 - 25 June 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to OECD guideline study. Acceptable, well documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

(adopted 1981)

Deviations:

yes

Remarks:

A test group consisting of 5 animals was treated by single gavage application with an aqueous solution of the test substance.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-[2-(dimethylamino)ethoxy]ethanol

EC Number:

216-940-1

EC Name:

2-[2-(dimethylamino)ethoxy]ethanol

Cas Number:

1704-62-7

Molecular formula:

C6H15NO2

IUPAC Name:

2-[2-(dimethylamino)ethoxy]ethan-1-ol

Details on test material:

- Name of test material (as cited in study report): Lupragen N 107
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Germany
- Weight at study initiation: male: 187 g (mean); female: 177 g (mean)
- Fasting period before study: 16 h before administration but water was available ad libitum
- Housing: 5 animals per cage
- Diet: KLIBA-Labordiaet 343, Klingentalmuehle AG, CH, ad libitum
- Water: tap water ad libitum
- Acclimation period: at least 1 week


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5 % aqueous CMC

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 50, 38.3, 21.5, and 10.0 % (w/v), respectively, for 5000, 3830, 2150, and 1000 mg/kg bw doses
- Amount of vehicle (if gavage): 10 mL/kg bw

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw

Doses:

1000, 2150, 3830 and 5000 mg/kg bw

No. of animals per sex per dose:

5 animals/sex/dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: recording of signs and symptoms several times on the day of administration, at least once each workday. Check for moribund and dead animals twice each workday and once on holidays.
- Frequency of weighing: days 0, 5, 7, 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 2 150 - <= 3 830 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

>= 2 150 - <= 3 830 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 2 150 mg/kg bw

Based on:

test mat.

Mortality:

See details in "remarks on results".

Clinical signs:

other: See details in "remarks on results".

Gross pathology:

- Animals that died:
General congestion.
Stomach/small intestines: mucosa reddened, bloody contents.

- Sacrificed animals:
No pathologic findings noted.

Any other information on results incl. tables

Mortality:

Dose (mg/kg bw)	Conc.	Gender	1 h	24 h	48 h	day 7	day 14
5000	50	male	0/5	5/5	5/5	5/5	5/5
5000	50	female	0/5	5/5	5/5	5/5	5/5
3830	38.3	male	0/5	5/5	5/5	5/5	5/5
3830	38.3	female	0/5	5/5	5/5	5/5	5/5
2150	21.5	male	0/5	0/5	0/5	0/5	0/5
2150	21.5	female	0/5	2/5	2/5	2/5	2/5
1000	10	male	0/5	0/5	0/5	0/5	0/5
1000	10	female	0/5	0/5	0/5	0/5	0/5

Weight (g):

Dose (mg/kg bw)	Gender	day 0	day 5	day 7	day 13
5000	male	92	-	-	-
5000	female	179	-	-	-
3830	male	186	-	-	-
3830	female	172	-	-	-
2150	male	179	-	238	272
2150	female	179	-	197	215
1000	male	189	244	-	291
1000	female	180	205	-	217

Clinical signs:

Dose (mg/kg bw)	5000		3830		2150		1000
	male	female	male	female	male	female	male	female
Dyspnea	1 h - 4 h	1 h - 4 h	30 m - 5 h	30 m - 5 h	1 d - 2 d	4 h - 2 d	-	-
Apathy	1 h - 4 h	1 h - 4 h	30 m - 5 h	30 m - 5 h	1 d - 2 d	4 h - 2 d	-	-
Abnormal position	4 h	4 h	-	4 h - 5 h	-	-	-	-
Staggering	4 h	4 h	30 m - 5 h	30 m - 1 h	-	1 d - 2 d	-	-
Atonia	4 h	4 h	-	4 h - 5 h	-	-	-	-
Paresis	4 h	4 h	-	4 h - 5 h	-	-	-	-
Piloerection	1 h - 4 h	1 h - 4 h	30 m - 5 h	30 m - 5 h	1 d - 2 d	4 h - 2 d	-	-
Cyanosis	4 h	4 h	4 h - 5 h	4 h - 5 h	-	-	-	-
Exsiccosis	4 h	4 h	-	4 h- 5 h	-	-	-	-
Salivation	4 h	4 h	-	-	-	-	-	-
Poor general state	1 h - 4 h	1 h - 4 h	30 m - 5 h	30 m - 5 h	1 d - 2 d	4 h - 2 d	-	-

min: minutes

h: hour

d: day

The test substance caused systemic toxicity (including mortality) in a dose dependent manner.