1,2,4-triazole

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study (OECD 402)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

reliability scoring based on 1987 guideline

Deviations:

yes

Remarks:

Source, purity, chemical characteristics of test substance, temperature, light cycle, and humidity not provided; grading scale not provided

GLP compliance:

no

Remarks:

study pre-dates GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2,4-triazole

EC Number:

206-022-9

EC Name:

1,2,4-triazole

Cas Number:

288-88-0

Molecular formula:

C2H3N3

IUPAC Name:

1H-1,2,4-triazole

Details on test material:

- Name of test material (as cited in study report): 1,2,4-Triazole
- Analytical purity: reoprted as technically pure

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: from the breeder Winkelmann (Borchen)
- Age at study initiation: no data
- Weight at study initiation: 160-180g
- Fasting period before study: no data
- Housing: in tyupe-III Makrolon cages
- Diet (e.g. ad libitum): Altromin R standard diet, ad libitum
- Water (e.g. ad libitum): not precised, ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS: no data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

other: Cremophor EL

Details on dermal exposure:

Test substance was applied to shaven dorsal skin. After 24 hours of application, the skin was washed with water and soap.

Duration of exposure:

24 hours

Doses:

1000, 2000 (females only), 2500, 3500, 4000 (females), 5000 mg/kg body weight

No. of animals per sex per dose:

1000 (5 males; 5 females), 2000 (10 females), 2500 (10 males; 10 females), 3500 (10 males; 10 females), 4000 (10 females), 5000 (10 males; 20 females)

Control animals:

not required

Details on study design:

14 day observation period

Statistics:

The calculation of the mean lethal dose (LD50) in carried out using probit analysis.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

4 200 mg/kg bw

95% CL:

> 3 081 - < 5 725

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

3 129 mg/kg bw

95% CL:

> 2 203 - < 3 648

Mortality:

Yes

Clinical signs:

other: The animals exhibited; reduction in general well-being, accompanied by sedation and breathing disorders. At higher doses, the animals were lying in the abdominal or side position. The symptoms appeared within an hour of administration and were observed fo

Gross pathology:

Gross pathology examination of the animals revealed no major changes.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: CLP (EC1272/2008)

Executive summary:

A guideline study (OECD 402) was performed in rats to calculate LD50 in dermal route. Rats were exposed to 1,2,4 -triazole at 1000, 2000 (females only), 2500, 3500, 4000 (females), 5000 mg/kg body weight (a single exposure), and were observed during 14 days. The mortality of each animal was reported and the LD50 was calculated. The LD50 of 1,2,4-triazole is 4200 mg/kg bw in male rats, and 3129 mg/kg bw in female rats.

1,2,4 -triazole is not classified for acute dermal toxicity according to the regulation EC no.1272/2008 (CLP).