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EC number: 259-910-3 | CAS number: 55934-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline 471
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- [(butoxymethylethoxy)methylethoxy]propan-1-ol
- EC Number:
- 259-910-3
- EC Name:
- [(butoxymethylethoxy)methylethoxy]propan-1-ol
- Cas Number:
- 55934-93-5
- Molecular formula:
- C13H28O4
- IUPAC Name:
- 1-[2-(2-butoxy-1-methylethoxy)-1-methylethoxy]propan-1-ol
- Details on test material:
- - Name of test material (as cited in study report): Ttripropylene glycol-n-butyl ether
- Molecular formula (if other than submission substance): C13H28O4
- Molecular weight (if other than submission substance): 248
- Physical state: liquid
- Analytical purity:96.12%
- Impurities (identity and concentrations):
- Lot/batch No.: EB880621R004
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver homogenate
- Test concentrations with justification for top dose:
- 50, 158, 500, 1580, & 5000.0 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2 aminoanthramine for all the strains in the absence of S9, sodium azide-S9TA 100 & TA 1535, 2NF -S9 in TA 100, 2AA -S9 in TA 1537
- Details on test system and experimental conditions:
- Type: Ames test
METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 30 min
- Incubation period: 48 hours
NUMBER OF REPLICATIONS:
3 replicates per dose
DETERMINATION OF CYTOTOXICITY
- Method: bacterial background lawn evaluation
- Evaluation criteria:
- Concentrations of the test chemical which are overtly toxic are easily visualized as showing no bacterial growth on the plate (i.e. absence of background lawn). Lower levels of toxicity may be seen as a thin or sparse bacterial lawn, a reduction in the number of revertants, or the appearance of microcolonies (overgrown background lawn). Positive and negative controls were run concurrently with the test chemical, and appropriate responses for these controls are prerequesites for evaluating the response of the bacteria to the test chemical.
A test chemical is considered a bacterial mutagen if both the mean number of revertant colonies observed is at least three times higher than the mean of the negative (solvent) control and at the same time it produces a dose response relationship over several concentrations. If a chemical produces reproducible reversion rates in excess of 3x over background, but no definitive dose response relationship, it is considered to be a presumptive bacterial mutagen. If a chemical produces reproducible reversion rates greater than 2x but less than 3x over the negative controls, the results are considered to be equivocal or inconclusive. Test substances failing to meet the above criteria are considered non-mutagenic in this system.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
RANGE-FINDING/SCREENING STUDIES: was conducted
COMPARISON WITH HISTORICAL CONTROL DATA: was done
ADDITIONAL INFORMATION ON CYTOTOXICITY:no cytotoxicity was observed- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
see the attached word doc for tables.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results
negative
DOWANOL TPnB did not induce a mutagenic response in any of the tester strains as judged by the frequency of histidine-independent (his+) revertants. Hence, DOWANOL TPnB was classified as negative in the Ames test under the experimental conditions used. - Executive summary:
TriPropylene glycol- n-butyl ether was evaluated in the Salmonella/mammalian-microsome bacterial mutagenicity assay (Ames test) using a pre-incubation modification of the standard assay. The test was conducted both in the presence and in the absence of externally supplied metabolic activation system. The test was conducted using Salmonella typhimurium bacetrial tester strains TA98, TA100, TA1535 and TA1537. The test agent was initially assayed in TA100 at concentrations of 0 (solvent control), 5.0, 15.8, 50.0, 158.0, 500.0, 1580.0 and 5000.0 micrograms/plate. The test material was assayed at least two times in each tester strain up to a maximum concentration of 5000 ug/plate.
In addition to the mutation in the histidine operon, these tester strains contain other mutations that increase their ability to detect mutagens.
Based upon the results in TA100, the top five concentrations were repeated in TA98, TA100, TA1535 and TA1537. The assay was repeated independently at the same five concentrations using all four tester strains. The test material did not induce a mutagenic response in any of the tester strains in either of the assays as judged by the frequencies of histidine-independent (his+) revertants. Hence, the test material was classified as negative in the under the experimental conditions used.
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