Diethyl carbonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

study plan dated 2012-01-13, experimental starting date 2012-01-31, experimental completion date 2012-02-02, draft report dated 2012-02-17, final report dated 2012-03-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Test organisms (species):

other: Daphnia magna STRAUS

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.502 mmol/L;
250 mg CaCO3/L

Test temperature:

20.2-20.9 °C

pH:

7.4-7.7

Dissolved oxygen:

stayed above 8.7 mg/L throughout the test

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CL not determinable

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CL not determinable

The correlation between measured and nominal concentration was very good (103 % recovery of the start concentration after 48h). Therefore the determination of the biological results was based on the nominal concentration. In the control and treatment, none of the daphnia died or showed any signs of abnormal behaviour throughout the test (NOEC 24 h, 48 h ≥ 100 mg/L, 24 h, 48 h EC100i > 100 mg/L). In the positive control group (K2Cr2O7) immobility was 15 % at a concentration of 1.0 mg/L and 100 % at 3.0 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The toxic potential of the test substance diethyl carbonate towards Daphnia magna STRAUS was evaluated in this study. The main study was performed as a limit test at 100 mg/L. Twenty daphnia were exposed to the test substance for 48 hours in a static, closed test system. Counts of daphnia after 24 and 48 hours showed no immobilisation in the treatment group. The determined 24h- and 48h-NOEC for the test substance diethyl carbonate was ≥ 100 mg/L.

Description of key information

Determined NOEC in short-term-toxicity study to daphnia was ≥ 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The authors of the short-term-toxicity study on Daphnia (48 h) evaluated for the test substance diethyl carbonate a NOEC ≥ 100 mg/L. Based on this result the author of this IUCLID data set assumed that the corresponding EC50 should be >> 100 mg/L (much greater than 100 mg/L).