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The acute toxicity of the test item Squalane to Zebra fish, Daphnia magna and green algal species was determined according to the OECD Guidelines No. 203, 202 and 201 respectively. The results indicated that the test item Squalane had no toxic effects up to a loading rate of 100 mg/L (mean measured concentration of 3.9°μg/L, 3.8°μg/L or 7.5 µg/L respectively for OECD test n°203, 202 and 201) and therefore up to its solubility limit in the test water under the tests conditions.