tert-butyl 3,5,5-trimethylperoxyhexanoate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.2 (Acute Toxicity (Inhalation))

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

tert-butyl 3,5,5-trimethylperoxyhexanoate

EC Number:

236-050-7

EC Name:

tert-butyl 3,5,5-trimethylperoxyhexanoate

Cas Number:

13122-18-4

Molecular formula:

C13H26O3

IUPAC Name:

tert-butyl 3,5,5-trimethylhexaneperoxoate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Central Institute for the Breeding of Laboratory Animals TNO, Zeist, The Netherlands
- Age at study initiation: Young adult
- Weight at study initiation: Males: about 235 g, females: about 160 g
- Housing: stainless steel/ glass exposure chamber; five male and five female rats in separate wire screen cages

Administration / exposure

Route of administration:

inhalation: mist

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: stainless steel/ glass aerodynamic nozzle nebulizer
- Exposure chamber volume: 1.5 m3
- Airflow rate: 3 m3/ h

TEST ATMOSPHERE
- Brief description of analytical method used: Samples of the atmosphere were drawn through a Bergshoeff air-sampler filled with xylene and subsequently analysed by gas-liquid chromatography with the aid of an Intersmat gaschromatograph with a QF-1 (4.8% diglycerol on a chromosorb G-AW-DMCS) stainless-steel column.
- Samples taken from breathing zone: yes

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

No data

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: no

Statistics:

not performed

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.8 mg/L air

Exp. duration:

4 h

Mortality:

No mortality occurred

Other findings:

During the first half hour of the exposure period the rats were somewhat restless. This symptom gradually disappeared and after one hour all rats were asleep. This situation continued throughout the remainder of the exposure period.
Since no mortality occurred during the 4 hours of the exposure and the subsequent observation period, the 4 hour LC 50 could not be determined.

Applicant's summary and conclusion

Interpretation of results:

not classified

Conclusions:

The results of the present acute inhalation study lead to the conclusion that the 4-hour LC 50 of tert-Butylperoxy-3,5,5-trimethylhexanoat is higher than 0.8 mg/L of air.

Executive summary:

The acute inhalation toxicity of tert-Butylperoxy-3,5,5-trimethylhexanoat was studied by exposing rats for 4 hours to an aerosol of the undiluted substance at a concentration of 0.8 mg/L of air.

No mortality occurred and no signs of intoxication were observed.

It was concluded that the 4 -hour LC 50 of tert-butyl 3,5,5-trimethylperoxyhexanoate was higher than 0.8 mg/L of air.