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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: 84/449/EEC (OJ No L251)
Deviations:
no
GLP compliance:
yes
Test type:
fixed dose procedure
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[2-(acryloyloxy)ethyl]trimethylammonium chloride
EC Number:
256-176-6
EC Name:
[2-(acryloyloxy)ethyl]trimethylammonium chloride
Cas Number:
44992-01-0
Molecular formula:
C8H16NO2.Cl
IUPAC Name:
Trimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride
Details on test material:
Test substance: other TS: ADAMQUAT MC 80
Source: Norsolor
Batch number: no data
Purity: no data
Other: Test substance was supplied as an 80% aqueous solution.

Test animals

Species:
rat
Strain:
other: CD [Crl: CD (SD) BR VAF plus]
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River UK (Margate, Kent, UK)
- Age at study initiation: 7 to 10 weeks
- Weight at study initiation: 200 - 300 g

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: occlusive
- % coverage: 10%
REMOVAL OF TEST SUBSTANCE
- Washing (if done): area of skin was washed with warm water (30-40°C) and then blotted dry with absorbent paper
- Time after start of exposure:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.75 ml/kg bw
- Concentration (if solution): as supplied: 80% in water
Duration of exposure:
24 hours
Doses:
2000 mg/kg (2000 g of 80% solution / kg bw)
Total volume applied: 1.75 ml/kg, according to the body weight determined on the day of treatment and test substance specific gravity (1.14)
No. of animals per sex per dose:
5 males and 5 females
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
Observations:
-Clinical signs: examined at least once a day;
-Mortality: recorded at least once a day
- Body weight: measured just before administration then on days 8 and 15.
- Necropsy:
- macroscopic examination of the main organs of the abdominal and thoracic cavities.
- microscopic examination: no
Statistics:
none

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD0
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No deaths occurred during the study.
Clinical signs:
other: No systemic effects were observed. Dose site irritation included slight erythema in all male rats on day 2 and slight to moderate erythema with slight to well-defined oedema among females from day 2 to day 7; the irritation for one female was limited to s
Gross pathology:
No macroscopic abnormalities were revealed at necropsy.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute LD0 (and LD50) of ADAMQUAT MC in the rat was determined to be greater than 2000 mg/kg body weight by the dermal route.