Dodecanedioic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Abstract, test duration 24 h instead of 48 h.

Principles of method if other than guideline:

Method: DIN 38412 part 11

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

- Dispersion: saturated solution

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Daphnia magna
- Strain: Daphnia magna (Strauss)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

none

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 27.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 27.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Test parameter: immobilization
- Behavioural abnormalities: no

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 (24h) based on nominal concentration was determined as > 27.6 mg/L indicating that the test substance is not harmful to aquatic invertebrates.

Executive summary:

An acute toxicity study with Daphnia magna was performed according to the DIN 38412 part 11 method, under static conditions (24 h incubation period). An analytical monitoring was included. No toxic reference or control treatment group was included in the test. There was no immobilization, toxic effects or abnormal responses. Thus the EC₅₀ (24 h) was >27.6 mg dodecanedioic acid /L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No analytic was performed. Nominal test concentrations exceeded the solubility of the test item, test duration 24 h instead of 48 h.

Principles of method if other than guideline:

Static screening method

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Daphnia magna (Strauss)
- Strain: strain identical with the strain of the Bundesgesundheitsamt/Inst. Wasser-Boden-Luft
- Source: own breed
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Post exposure observation period:

none

Hardness:

about 14 ºdH

Test temperature:

Room temperature

Nominal and measured concentrations:

Nominal: 1000 mg/l

Details on test conditions:

- Test vessel: glass beaker, covered with glass plate, 20 ml liquid volume
- Number of daphnids per vessel: about 20
- Photoperiod: about 16 hours illumination per day
- Food type: Chlorella kessleri
- Number of replicates: 1

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Test parameter: immobilization
- Behavioural abnormalities: no

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 (24h) based on nominal concentration was determined as > 1000 mg/L indicating that the test substance is not harmful to aquatic invertebrates.

Executive summary:

An acute toxicity study with Daphnia magna was performed according to the screening method (differing from OECD-Guideline 202, part 1 in: number of daphnids per concentration and fact that the substance concentration was not followed by chemical analysis), under static conditions (24 h incubation period). About 20 daphnids in one replicate were exposed to 1000 mg dodecanedioic acid /L nominal concentration. No toxic reference or control treatment group was included in the test. No analytical monitoring was included. There was no immobilization, toxic effects or abnormal responses. Thus the EC₅₀ (24 h) was >1000 mg dodecanedioic acid /L.

Description of key information

An acute daphnia test (24 h) conducted by Scholz N. (1988) studied the toxicity of dodecanedioic acid on Daphnia magna (determined in a static test according to the DIN 38412 part 11 method). The second acute toxicity study conducted by Wind T. and Mühlberg B. (1982) with Daphnia magna (about 20 daphnids in 1000 mg dodecanedioic acid /L nominal concentration) was performed according to the screening method (differing from OECD-Guideline 202), under static conditions (24 h incubation period) with one replicate per concentration level. 

Additional information

The results are summarised in the following table:

Species	Endpoint (exposure duration)	Exposure concentration	Reference
Daphnia magna	EC50 (24 h)	> 27.6 mg/L	Scholz N. (1988)
Daphnia magna	EC50 (24 h)	> 1000 mg/L	Wind T., Mühlberg B. (1982)

The available studies indicate that dodecanedioic acid concentrations up to 1000 mg/L are not toxic to aquatic invertebrates. However, taking into considaration that no analytical monitoring was conducted and that the water solubilty is between 30 mg/L and 40 mg/L the endpoint that should be considered for risk assessment purposes is >27.6 mg/L.