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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Several data on acute and chronic toxicity of methanol to fish, invertebrates, and algae are available:


Acute Toxicity

LC50 (96h) = 28100 mg/L Pimephales promelas

LC50 (96h) = 20100 mg/L Oncorhynchus mykiss (=Salmo gairdneri)

LC50 (96h) = 15400 mg/L Lepomis macrochirus

EC50 (48h) > 10000 mg/L Daphnia magna

EC50 (96h) ca. 22000 mg/L Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata)


Chronic Toxicity

NOEC (200h) = 7900 - 15800 mg/L Oryzias latipes



EC 50: 19800 mg/L activated sludge

IC50: >1000 mg/L avtivated sludge

IC50: 880 mg/L Nitrosamonas

toxic limit concentration: 530 - 6600 mg/L Pseudomonas, Microcystis aeruginosa.


The results indicate a very low acute toxicity for aquatic organisms, well above 10000 mg/L. Also for microorganisms data indicate a low toxicity. The PNEC for aquatic organisms was derived from the LC50 (96h) = 15400 mg/L Lepomis macrochirus using an assessment factor of 100. The choice of this assessment factor is based on the very low acute toxicity of methanol in all throphic levels and its unspecific mode of action (non-polar narcosis). Although the available information on long-term toxicity in fish (Gonzales-Doncel et al., 2008) is not used to derive the PNEC, the reported No Observed Effect Concentration of

7900 - 15800 mg/L in Oryzias latipes confirms the low toxicity of methanol also after chronic exposure.

This is also supported by available tests on chronic toxicity for Daphnids conducted with the structurally related substances propan-2 -ol and butan 1 -ol (NOECs respectively of > 100 mg/L and 18 mg/L).